ArticleJournal of neuroscience methods2016
Semi-automated rapid quantification of brain vessel density utilizing fluorescent microscopy.
Article in Journal of neuroscience methods, 2016. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 14 papers.
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
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Who cites it
14 citing papers in PubMed, 29 citations in OpenAlex.
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- Brain oxygen responses induced by arousing stimuli and fentanyl: generalized or structure-specific?American journal of physiology. Regulatory, integrative and comparative physiology · 2026Article
- Q-VAT: Quantitative Vascular Analysis Tool.Frontiers in cardiovascular medicine · 2023Article
- Quantitative cerebrovascular reactivity MRI in mice using acetazolamide challenge.Magnetic resonance in medicine · 2022Article
- Activation of Glutamate Transport Increases Arteriole DiameterFrontiers in cellular neuroscience · 2022Article
- More than just summed neuronal activity: how multiple cell types shape the BOLD response.Philosophical transactions of the Royal Society of London. Series B, Biological sciences · 2021Review
- Nanoparticles Containing a Combination of a Drug and an Antibody for the Treatment of Breast Cancer Brain Metastases.Molecular pharmaceutics · 2020Article
- Sono-optogenetics facilitated by a circulation-delivered rechargeable light source for minimally invasive optogenetics.Proceedings of the National Academy of Sciences of the United States of America · 2019Article
- Lab-On-A-Chip for the Development of Pro-/Anti-Angiogenic Nanomedicines to Treat Brain Diseases.International journal of molecular sciences · 2019Review
- Improving CNS Delivery to Brain Metastases by Blood-Tumor Barrier Disruption.Trends in cancer · 2019Review
- Increased cerebral vascularization and decreased water exchange across the blood-brain barrier in aquaporin-4 knockout mice.PloS one · 2019Article
- Permeability changes and effect of chemotherapy in brain adjacent to tumor in an experimental model of metastatic brain tumor from breast cancer.BMC cancer · 2018Article
- Liposomal Irinotecan Accumulates in Metastatic Lesions, Crosses the Blood-Tumor Barrier (BTB), and Prolongs Survival in an Experimental Model of Brain Metastases of Triple Negative Breast Cancer.Pharmaceutical research · 2018Article
Corrections and comments
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Authors and funding
9 authors at 4 institutions in 2 countries.
Funding
Abstract
backgroundMeasurement of vascular density has significant value in characterizing healthy and diseased tissue, particularly in brain where vascular density varies among regions. Further, an understanding of brain vessel size helps distinguish between capillaries and larger vessels like arterioles and venules. Unfortunately, few cutting edge methodologies are available to laboratories to rapidly quantify vessel density. NEW
methodWe developed a rapid microscopic method, which quantifies the numbers and diameters of blood vessels in brain. Utilizing this method we characterized vascular density of five brain regions in both mice and rats, in two tumor models, using three tracers.
resultsWe observed the number of sections/mm(2) in various brain regions: genu of corpus callosum 161±7, hippocampus 266±18, superior colliculus 300±24, frontal cortex 391±55, and inferior colliculus 692±18 (n=5 animals). Regional brain data were not significantly different between species (p>0.05) or when using different tracers (70kDa and 2000kDa Texas Red; p>0.05). Vascular density decreased (62-79%) in preclinical brain metastases but increased (62%) a rat glioma model. COMPARISON WITH EXISTING
methodsOur values were similar (p>0.05) to published literature. We applied this method to brain-tumors and observed brain metastases of breast cancer to have a ∼2.5-fold reduction (p>0.05) in vessels/mm(2) compared to normal cortical regions. In contrast, vascular density in a glioma model was significantly higher (sections/mm(2) 736±84; p<0.05).
conclusionsIn summary, we present a vascular density counting method that is rapid, sensitive, and uses fluorescence microscopy without antibodies.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.