ArticleThe journal of physical chemistry letters2016
Saccharides as Prospective Immobilizers of Nucleic Acids for Room-Temperature Structural EPR Studies.
Article in The journal of physical chemistry letters, 2016. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 4 papers.
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Who cites it
4 citing papers in PubMed, 22 citations in OpenAlex.
- Blatter-Radical-Grafted Mesoporous Silica as Prospective Nanoplatform for Spin Manipulation at Ambient Conditions.Angewandte Chemie (International ed. in English) · 2021Article
- In-Cell Trityl-Trityl Distance Measurements on Proteins.The journal of physical chemistry letters · 2020Article
- Mapping Magnetic Properties and Relaxation in Vanadium(IV) Complexes with Lanthanides by Electron Paramagnetic Resonance.Molecules (Basel, Switzerland) · 2019Article
- Triarylmethyl Radicals: EPR Study ofZeitschrift fur physikalische Chemie (Frankfurt am Main, Germany) · 2017Article
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Authors and funding
7 authors at 5 institutions in 1 country.
Funding
Abstract
Pulsed dipolar electron paramagnetic resonance (EPR) spectroscopy is a powerful tool for structural studies of biomolecules and their complexes. This method, whose applicability has been recently extended to room temperatures, requires immobilization of the studied biosystem to prevent averaging of dipolar couplings; at the same time, the modification of native conformations by immobilization must be avoided. In this work, we provide first demonstration of room-temperature EPR distance measurements in nucleic acids using saccharides trehalose, sucrose, and glucose as immobilizing media. We propose an approach that keeps structural conformation and unity of immobilized double-stranded DNA. Remarkably, room-temperature electron spin dephasing time of triarylmethyl-labeled DNA in trehalose is noticeably longer compared to previously used immobilizers, thus providing a broader range of available distances. Therefore, saccharides, and especially trehalose, can be efficiently used as immobilizers of nucleic acids, mimicking native conditions and allowing wide range of structural EPR studies at room temperatures.
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Registered trials
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