ArticleDevelopmental biology2017
Exploring the functions of nonclassical MHC class Ib genes in Xenopus laevis by the CRISPR/Cas9 system.
Article in Developmental biology, 2017. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 19 papers.
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Who cites it
19 citing papers in PubMed.
- Review
- The Amphibian Major Histocompatibility Complex-A Review and Future Outlook.Journal of molecular evolution · 2025Review
- The Amphibian Genomics Consortium: advancing genomic and genetic resources for amphibian research and conservation.BMC genomics · 2024Review
- The Amphibian Genomics Consortium: advancing genomic and genetic resources for amphibian research and conservation.bioRxiv : the preprint server for biology · 2024Article
- Revealing mitf functions and visualizing allografted tumor metastasis in colorless and immunodeficient Xenopus tropicalis.Communications biology · 2024Article
- Advances in the Xenopus immunome: Diversification, expansion, and contraction.Developmental and comparative immunology · 2023Review
- Environmental endocrine disruptors and amphibian immunity: A bridge between the thyroid hormone axis and T cell development.Developmental and comparative immunology · 2023Article
- γδ T, NKT, and MAIT Cells During Evolution: Redundancy or Specialized Functions?Journal of immunology (Baltimore, Md. : 1950) · 2022Review
- Optimization of CRISPR/Cas9-mediated gene disruption in Xenopus laevis using a phenotypic image analysis technique.Development, growth & differentiation · 2022Article
- Coevolution between MHC Class I and Antigen-Processing Genes in Salamanders.Molecular biology and evolution · 2021Article
- Ttc30a affects tubulin modifications in a model for ciliary chondrodysplasia with polycystic kidney disease.Proceedings of the National Academy of Sciences of the United States of America · 2021Article
- A Highly Complex, MHC-Linked, 350 Million-Year-Old Shark Nonclassical Class I Lineage.Journal of immunology (Baltimore, Md. : 1950) · 2021Article
- Evolutionary Underpinnings of Innate-Like T Cell Interactions with Cancer.Immunological investigations · 2019Review
- Review
- Distinct MHC class I-like interacting invariant T cell lineage at the forefront of mycobacterial immunity uncovered inProceedings of the National Academy of Sciences of the United States of America · 2018Article
- Frog's DCs have it all in one.European journal of immunology · 2018Article
- Tissue-Specific Gene Inactivation inGenetics · 2018Article
- Tumor immunology viewed from alternative animal models-theCurrent pathobiology reports · 2017Article
- A frog's view of EphrinB signaling.Genesis (New York, N.Y. : 2000) · 2017Review
Corrections and comments
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Authors and funding
3 authors.
Funding
Abstract
A large family of highly related and clustered Xenopus nonclassical MHC class Ib (XNC) genes influences Xenopus laevis immunity and potentially other physiological functions. Using RNA interference (RNAi) technology, we previously demonstrated that one of XNC genes, XNC10.1, is critical for the development and function of a specialized innate T (iT) cell population. However, RNAi limitation such as a variable and unstable degree of gene silencing in F0 and F1 generations is hampering a thorough functional analysis of XNC10.1 and other XNC genes. To overcome this obstacle, we adapted the CRISPR/Cas9-mediated gene editing technique for XNC genes. We efficiently and specifically generated single gene knockouts of XNC10.1, XNC11, and XNC1 as well as double gene knockouts of XNC10.1 and XNC11 in X. laevis. In single XNC10.1 knockout X. laevis tadpoles, the absence of XNC10.1 and Vα6-Jα1.43 invariant T cell receptor rearrangement transcripts indicated XNC10.1 loss-of-function and deficiency in Vα6-Jα1.43 iT cells. Notably, targeting XNC10.1 did not affect neighboring XNC genes exhibiting high sequence similarity. Furthermore, XNC1 gene disruption induced mortality during developmental stage 47, suggesting some non-immune but essential function of this gene. These data demonstrate that the CRISPR/Cas9 system can be successfully adapted for genetic analysis in F0 generation of X. laevis.
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