ArticlePlant biotechnology journal2017
Ice-binding proteins confer freezing tolerance in transgenic Arabidopsis thaliana.
Article in Plant biotechnology journal, 2017. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 14 papers.
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Who cites it
14 citing papers in PubMed, 38 citations in OpenAlex.
- Hyperactive ice-binding proteins stabilize cell membranes and improve resistance to dehydration stress in Caenorhabditis elegans.FEBS open bio · 2026Article
- Integrated metabolomics and transcriptomics analysis during seed germination of waxy corn under low temperature stress.BMC plant biology · 2023Article
- Coping with the cold: unveiling cryoprotectants, molecular signaling pathways, and strategies for cold stress resilience.Frontiers in plant science · 2023Review
- Article
- Anti-icing strategies of plant surfaces: the ice formation on leaves visualized by Cryo-SEM experiments.Die Naturwissenschaften · 2022Article
- Antifreeze protein from Ammopiptanthus nanus functions in temperature-stress through domain A.Scientific reports · 2021Article
- Article
- Ice Binding Proteins: Diverse Biological Roles and Applications in Different Types of Industry.Biomolecules · 2020Review
- Drought and heat stress-related proteins: an update about their functional relevance in imparting stress tolerance in agricultural crops.TAG. Theoretical and applied genetics. Theoretische und angewandte Genetik · 2019Review
- Both cold and sub-zero acclimation induce cell wall modification and changes in the extracellular proteome in Arabidopsis thaliana.Scientific reports · 2019Article
- Identification of Plant Ice-binding Proteins Through Assessment of Ice-recrystallization Inhibition and Isolation Using Ice-affinity Purification.Journal of visualized experiments : JoVE · 2017Article
- Ice-Binding Proteins in Plants.Frontiers in plant science · 2017Review
- Transcriptome Sequencing Identified Genes and Gene Ontologies Associated with Early Freezing Tolerance in Maize.Frontiers in plant science · 2016Article
- Knockdown of Ice-Binding Proteins in Brachypodium distachyon Demonstrates Their Role in Freeze Protection.PloS one · 2016Article
Corrections and comments
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Authors and funding
3 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Lolium perenne is a freeze-tolerant perennial ryegrass capable of withstanding temperatures below -13 °C. Ice-binding proteins (IBPs) presumably help prevent damage associated with freezing by restricting the growth of ice crystals in the apoplast. We have investigated the expression, localization and in planta freezing protection capabilities of two L. perenne IBP isoforms, LpIRI2 and LpIRI3, as well as a processed IBP (LpAFP). One of these isoforms, LpIRI2, lacks a conventional signal peptide and was assumed to be a pseudogene. Nevertheless, both LpIRI2 and LpIRI3 transcripts were up-regulated following cold acclimation. LpIRI2 also demonstrated ice-binding activity when produced recombinantly in Escherichia coli. Both the LpIRI3 and LpIRI2 isoforms appeared to accumulate in the apoplast of transgenic Arabidopsis thaliana plants. In contrast, the fully processed isoform, LpAFP, remained intracellular. Transgenic plants expressing either LpIRI2 or LpIRI3 showed reduced ion leakage (12%-39%) after low-temperature treatments, and significantly improved freezing survival, while transgenic LpAFP-expressing lines did not confer substantial subzero protection. Freeze protection was further enhanced by with the introduction of more than one IBP isoform; ion leakage was reduced 26%-35% and 10% of plants survived temperatures as low as -8 °C. Our results demonstrate that apoplastic expression of multiple L. perenne IBP isoforms shows promise for providing protection to crops susceptible to freeze-induced damage.
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