ArticleNucleic acids research2016
Biochemical and genetic analysis of the role of the viral polymerase in enterovirus recombination.
Article in Nucleic acids research, 2016. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 27 papers.
What it found
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Who cites it
27 citing papers in PubMed, 47 citations in OpenAlex.
- Enterovirus C recombination groups: RNA sequence similarity and the viral polymerase underpin sexual replication mechanisms.Journal of virology · 2025Article
- Genomic Characterization of Two Bovine Enterovirus Strains Isolated from Newly Transported Cattle.Veterinary sciences · 2025Article
- Mechanism of Forced-Copy-Choice RNA Recombination by Enteroviral RNA-Dependent RNA Polymerases.ACS bio & med chem Au · 2025Article
- A Fluorescent Reporter Virus Toolkit for Interrogating Enterovirus Biology and Host Interactions.Viruses · 2025Article
- Understanding neurotropic enteric viruses: routes of infection and mechanisms of attenuation.Cellular and molecular life sciences : CMLS · 2024Review
- A review of the recombination events, mechanisms and consequences of Coxsackievirus A6.Infectious medicine · 2024Review
- Article
- The enterovirus genome can be translated in an IRES-independent manner that requires the initiation factors eIF2A/eIF2D.PLoS biology · 2023Article
- Review
- Generated Randomly and Selected Functionally? The Nature of Enterovirus Recombination.Viruses · 2022Article
- Article
- Recombination in Positive-Strand RNA Viruses.Frontiers in microbiology · 2022Review
- Isolation and Identification of Inter-SpeciesViruses · 2021Article
- Emergence of a novel recombinant of CV-A5 in HFMD epidemics in Xiangyang, China.BMC medical genomics · 2021Article
- Induced intra- and intermolecular template switching as a therapeutic mechanism against RNA viruses.Molecular cell · 2021Article
- Imprecise recombinant viruses evolve via a fitness-driven, iterative process of polymerase template-switching events.PLoS pathogens · 2021Article
- Review
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- Article
- Review
Corrections and comments
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Authors and funding
4 authors at 2 institutions in 2 countries.
Funding
Abstract
Genetic recombination in single-strand, positive-sense RNA viruses is a poorly understand mechanism responsible for generating extensive genetic change and novel phenotypes. By moving a critical cis-acting replication element (CRE) from the polyprotein coding region to the 3' non-coding region we have further developed a cell-based assay (the 3'CRE-REP assay) to yield recombinants throughout the non-structural coding region of poliovirus from dually transfected cells. We have additionally developed a defined biochemical assay in which the only protein present is the poliovirus RNA dependent RNA polymerase (RdRp), which recapitulates the strand transfer events of the recombination process. We have used both assays to investigate the role of the polymerase fidelity and nucleotide turnover rates in recombination. Our results, of both poliovirus intertypic and intratypic recombination in the CRE-REP assay and using a range of polymerase variants in the biochemical assay, demonstrate that RdRp fidelity is a fundamental determinant of recombination frequency. High fidelity polymerases exhibit reduced recombination and low fidelity polymerases exhibit increased recombination in both assays. These studies provide the basis for the analysis of poliovirus recombination throughout the non-structural region of the virus genome and provide a defined biochemical assay to further dissect this important evolutionary process.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.