ArticleThe Journal of biological chemistry2016
Characterization of Anammox Hydrazine Dehydrogenase, a Key N2-producing Enzyme in the Global Nitrogen Cycle.
Article in The Journal of biological chemistry, 2016. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 38 papers.
What it found
Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.
The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.
Who cites it
38 citing papers in PubMed, 126 citations in OpenAlex.
- Origin and Evolution of Key Enzymes in the Anammox Pathway Revisited.Genome biology and evolution · 2026Article
- Planktonic anammox bacteria toward a better understanding of ecophysiological traits and harnessing the untapped potential as a bioresource.Bioprocess and biosystems engineering · 2026Review
- Genomics-based insights into the expanded diversity and adaptation strategies of hadal trench anammox bacteria.ISME communications · 2026Article
- Exploring reductive stress as a regulation approach foriScience · 2025Article
- Nitric oxide-forming nitrite reductases in the anaerobic ammonium oxidizer Kuenenia stuttgartiensis.FEBS open bio · 2025Article
- Physiological and transcriptomic response of enriched anammox culture upon elevated hydrazine exposure.Biodegradation · 2025Article
- Age, metabolisms, and potential origin of dominant anammox bacteria in the global oxygen-deficient zones.ISME communications · 2024Article
- Microbial divergence and evolution. The case of anammox bacteria.Frontiers in microbiology · 2024Article
- Function and distribution of nitrogen-cycling microbial communities in the Napahai plateau wetland.Archives of microbiology · 2023Article
- Nitrogen Journey in Plants: From Uptake to Metabolism, Stress Response, and Microbe Interaction.Biomolecules · 2023Review
- "Applied and environmental microbiology · 2023Article
- Novel and unusual genes for nitrogen and metal cycling in Planctomycetota- and KSB1-affiliated metagenome-assembled genomes reconstructed from a marine subsea tunnel.FEMS microbiology letters · 2023Article
- Subsurface biogeochemical cycling of nitrogen in the actively serpentinizing Samail Ophiolite, Oman.Frontiers in microbiology · 2023Article
- Article
- Millimeter-scale vertical partitioning of nitrogen cycling in hypersaline mats reveals prominence of genes encoding multi-heme and prismane proteins.The ISME journal · 2022Article
- Microbiomes in the Challenger Deep slope and bottom-axis sediments.Nature communications · 2022Article
- Metabolic Diversity and Aero-Tolerance in Anammox Bacteria from Geochemically Distinct Aquifers.mSystems · 2022Article
- Nature's nitrite-to-ammonia expressway, with no stop at dinitrogen.Journal of biological inorganic chemistry : JBIC : a publication of the Society of Biological Inorganic Chemistry · 2022Review
- NHMicrobes and environments · 2022Article
- Specificity of SmallACS omega · 2021Article
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
11 authors at 4 institutions in 3 countries.
Funding
Abstract
Anaerobic ammonium-oxidizing (anammox) bacteria derive their energy for growth from the oxidation of ammonium with nitrite as the electron acceptor. N2, the end product of this metabolism, is produced from the oxidation of the intermediate, hydrazine (N2H4). Previously, we identified N2-producing hydrazine dehydrogenase (KsHDH) from the anammox organism Kuenenia stuttgartiensis as the gene product of kustc0694 and determined some of its catalytic properties. In the genome of K. stuttgartiensis, kustc0694 is one of 10 paralogs related to octaheme hydroxylamine (NH2OH) oxidoreductase (HAO). Here, we characterized KsHDH as a covalently cross-linked homotrimeric octaheme protein as found for HAO and HAO-related hydroxylamine-oxidizing enzyme kustc1061 from K. stuttgartiensis Interestingly, the HDH trimers formed octamers in solution, each octamer harboring an amazing 192 c-type heme moieties. Whereas HAO and kustc1061 are capable of hydrazine oxidation as well, KsHDH was highly specific for this activity. To understand this specificity, we performed detailed amino acid sequence analyses and investigated the catalytic and spectroscopic (electronic absorbance, EPR) properties of KsHDH in comparison with the well defined HAO and kustc1061. We conclude that HDH specificity is most likely derived from structural changes around the catalytic heme 4 (P460) and of the electron-wiring circuit comprising seven His/His-ligated c-type hemes in each subunit. These nuances make HDH a globally prominent N2-producing enzyme, next to nitrous oxide (N2O) reductase from denitrifying microorganisms.
Indexed as
Identifiers
What OpenQuestion holds
Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.