ReviewMicrobial cell factories2016
The fed-batch principle for the molecular biology lab: controlled nutrient diets in ready-made media improve production of recombinant proteins in Escherichia coli.
Review in Microbial cell factories, 2016. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 34 papers.
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Who cites it
34 citing papers in PubMed.
- Low inducer concentrations at 10 °C promotes soluble recombinant expression of Aedes aegypti mosquito midgut proteases in E. coli.Microbial cell factories · 2026Article
- Attenuated Bacteria-Based Tumor Therapy: Clinical Application Risks, Marketing Approval Restrictions, and Coping Strategies.Advanced science (Weinheim, Baden-Wurttemberg, Germany) · 2026Review
- Application of lactose based autoinduction for heterologous production of an active [NiFe] hydrogenase in E. coli.Biotechnology letters · 2025Article
- Optimization of fermentation conditions for the production of recombinant feruloyl esterase BpFaeFolia microbiologica · 2025Article
- Comparative evaluation of the extracellular production of a polyethylene terephthalate degrading cutinase by Corynebacterium glutamicum and leaky Escherichia coli in batch and fed-batch processes.Microbial cell factories · 2024Article
- Production Process Optimization of RecombinantACS omega · 2024Article
- Closing the loop: establishing an autonomous test-learn cycle to optimize induction of bacterial systems using a robotic platform.Frontiers in bioengineering and biotechnology · 2024Article
- Minimizing acetate formation from overflow metabolism inFrontiers in bioengineering and biotechnology · 2024Article
- An elevated OmpA expression during the production of a recombinant protein in Escherichia coli.Brazilian journal of microbiology : [publication of the Brazilian Society for Microbiology] · 2023Article
- High-throughput process development from gene cloning to protein production.Microbial cell factories · 2023Review
- β-glucosidase production by recombinant Pichia pastoris strain Y1433 under optimal feed profiles of fed-batch cultivation.Folia microbiologica · 2023Article
- Black glucose-releasing silicon elastomer rings for fed-batch operation allow measurement of the oxygen transfer rate from the top and optical signals from the bottom for each well of a microtiter plate.BMC biotechnology · 2023Article
- Machine learning-assisted medium optimization revealed the discriminated strategies for improved production of the foreign and native metabolites.Computational and structural biotechnology journal · 2023Article
- Implementation of a high cell density fed-batch for heterologous production of active [NiFe]-hydrogenase in Escherichia coli bioreactor cultivations.Microbial cell factories · 2022Article
- Implementation of Perforated Concentric Ring Walls Considerably Improves Gas-Liquid Mass Transfer of Shaken Bioreactors.Frontiers in bioengineering and biotechnology · 2022Article
- Expression of Bacillus amyloliquefaciens transglutaminase in recombinant E. coli under the control of a bicistronic plasmid system in DO-stat fed-batch bioreactor cultivations.Brazilian journal of microbiology : [publication of the Brazilian Society for Microbiology] · 2021Article
- Production of spiculisporic acid by Talaromyces trachyspermus in fed-batch bioreactor culture.Bioresources and bioprocessing · 2021Article
- Boosting Auto-Induction of Recombinant Proteins in Escherichia coli with Glucose and Lactose Additives.Protein and peptide letters · 2021Article
- Artificial Transcription Factors for Tuneable Gene Expression inFrontiers in bioengineering and biotechnology · 2021Article
- Strategies to Enhance Periplasmic Recombinant Protein Production Yields inFrontiers in bioengineering and biotechnology · 2021Review
Corrections and comments
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Authors and funding
3 authors.
Funding
No grant is acknowledged in the PubMed record.
Abstract
While the nutrient limited fed-batch technology is the standard of the cultivation of microorganisms and production of heterologous proteins in industry, despite its advantages in view of metabolic control and high cell density growth, shaken batch cultures are still the standard for protein production and expression screening in molecular biology and biochemistry laboratories. This is due to the difficulty and expenses to apply a controlled continuous glucose feed to shaken cultures. New ready-made growth media, e.g. by biocatalytic release of glucose from a polymer, offer a simple solution for the application of the fed-batch principle in shaken plate and flask cultures. Their wider use has shown that the controlled diet not only provides a solution to obtain significantly higher cell yields, but also in many cases folding of the target protein is improved by the applied lower growth rates; i.e. final volumetric yields for the active protein can be a multiple of what is obtained in complex medium cultures. The combination of the conventional optimization approaches with new and easy applicable growth systems has revolutionized recombinant protein production in Escherichia coli in view of product yield, culture robustness as well as significantly increased cell densities. This technical development establishes the basis for successful miniaturization and parallelization which is now an important tool for synthetic biology and protein engineering approaches. This review provides an overview of the recent developments, results and applications of advanced growth systems which use a controlled glucose release as substrate supply.
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