ReviewThe Journal of eukaryotic microbiology2016
Genome Editing by CRISPR/Cas9: A Game Change in the Genetic Manipulation of Protists.
Review in The Journal of eukaryotic microbiology, 2016. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 33 papers.
What it found
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
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Who cites it
33 citing papers in PubMed, 57 citations in OpenAlex.
- Glycosomal Phosphoenolpyruvate Carboxykinase CRISPR/Cas9-Deletion and Its Role in Trypanosoma cruzi Metacyclogenesis and Infectivity in Mammalian Host.FASEB journal : official publication of the Federation of American Societies for Experimental Biology · 2026Article
- Cloning-Free Genome Editing by CRISPR/T7RNAP/Cas9 in Trypanosoma cruzi.Methods in molecular biology (Clifton, N.J.) · 2026Article
- Review
- Next-Generation Leishmanization: Revisiting Molecular Targets for Selecting Genetically Engineered Live-AttenuatedMicroorganisms · 2023Review
- CRISPR-Cas9: Taming protozoan parasites with bacterial scissor.Journal of parasitic diseases : official organ of the Indian Society for Parasitology · 2022Review
- Ablation of theFrontiers in cellular and infection microbiology · 2022Article
- The long and winding road of reverse genetics inMicrobial cell (Graz, Austria) · 2021Article
- The Autophagy Machinery in Human-Parasitic Protists; Diverse Functions for Universally Conserved Proteins.Cells · 2021Review
- Deletion of a Golgi protein in Trypanosoma cruzi reveals a critical role for Mn2+ in protein glycosylation needed for host cell invasion and intracellular replication.PLoS pathogens · 2021Article
- Recent advances in genetic manipulation of Cryptosporidium.Current opinion in microbiology · 2020Review
- Rapid poxvirus engineering using CRISPR/Cas9 as a selection tool.Communications biology · 2020Article
- Computational approaches for drug discovery against trypanosomatid-caused diseases.Parasitology · 2020Review
- CRISPR/Cas9 Technology Applied to the Study of Proteins Involved in Calcium Signaling in Trypanosoma cruzi.Methods in molecular biology (Clifton, N.J.) · 2020Article
- A CRISPR/Cas9-riboswitch-Based Method for Downregulation of Gene Expression inFrontiers in cellular and infection microbiology · 2020Article
- State-of-the-art CRISPR/Cas9 Technology for Genome Editing in Trypanosomatids.The Journal of eukaryotic microbiology · 2019Review
- Advances in preclinical approaches to Chagas disease drug discovery.Expert opinion on drug discovery · 2019Review
- Emerging approaches and technologies in transplantation: the potential game changers.Cellular & molecular immunology · 2019Review
- CRISPR/Cas9-mediated one step bi-allelic change of genomic DNA in iPSCs and human RPE cells in vitro with dual antibiotic selection.Scientific reports · 2019Article
- Utilization of proliferable extracellular amastigotes for transient gene expression, drug sensitivity assay, and CRISPR/Cas9-mediated gene knockout in Trypanosoma cruzi.PLoS neglected tropical diseases · 2019Article
- Tissue Destruction Caused byCurrent clinical microbiology reports · 2019Review
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
3 authors at 1 institution in 2 countries.
Funding
Abstract
Genome editing by CRISPR (clustered regularly interspaced short palindromic repeats)/Cas9 (CRISPR-associated gene 9) system has been transformative in biology. Originally discovered as an adaptive prokaryotic immune system, CRISPR/Cas9 has been repurposed for genome editing in a broad range of model organisms, from yeast to mammalian cells. Protist parasites are unicellular organisms producing important human diseases that affect millions of people around the world. For many of these diseases, such as malaria, Chagas disease, leishmaniasis and cryptosporidiosis, there are no effective treatments or vaccines available. The recent adaptation of the CRISPR/Cas9 technology to several protist models will be playing a key role in the functional study of their proteins, in the characterization of their metabolic pathways, and in the understanding of their biology, and will facilitate the search for new chemotherapeutic targets. In this work we review recent studies where the CRISPR/Cas9 system was adapted to protist parasites, particularly to Apicomplexans and trypanosomatids, emphasizing the different molecular strategies used for genome editing of each organism, as well as their advantages. We also discuss the potential usefulness of this technology in the green alga Chlamydomonas reinhardtii.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.