ArticlePloS one2016
Accumulation of Poly(3-hydroxybutyrate) Helps Bacterial Cells to Survive Freezing.
Article in PloS one, 2016. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 28 papers.
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
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Who cites it
28 citing papers in PubMed, 95 citations in OpenAlex.
- Polyhydroxyalkanoates as multifaceted biopolymers: an eco-friendly alternative to conventional plastics.RSC advances · 2026Review
- Effect of SAM-Dependent Methyltransferases fromJournal of microbiology and biotechnology · 2025Article
- Evaluating stress resilience of cyanobacteria through flow cytometry and fluorescent viability assessment.Folia microbiologica · 2025Article
- Recent Advances in the Understanding of Stress Resistance Mechanisms in Probiotics: Relevance for the Design of Functional Food Systems.Probiotics and antimicrobial proteins · 2025Review
- A Review of Polyhydroxyalkanoates: Characterization, Production, and Application from Waste.Microorganisms · 2024Review
- Polyhydroxyalkanoate involvement in stress-survival of two psychrophilic bacterial strains from the High Arctic.Applied microbiology and biotechnology · 2024Article
- A widespread bacterial protein compartment sequesters and stores elemental sulfur.Science advances · 2024Article
- The Discovery of Membrane Vesicle Biogenesis in the Polyhydroxybutyrate-non-producing Mutant Strain of Cupriavidus necator H16.Microbes and environments · 2024Article
- Genetic engineering of low-temperature polyhydroxyalkanoate production by Acidovorax sp. A1169, a psychrophile isolated from a subglacial outflow.Extremophiles : life under extreme conditions · 2023Article
- The Role of Bacterial Polyhydroalkanoate (PHA) in a Sustainable Future: A Review on the Biological Diversity.International journal of environmental research and public health · 2023Review
- On the bioprotective effects of 3-hydroxybutyrate: Thermodynamic study of binary 3HB-water systems.Biophysical journal · 2023Article
- Article
- Glycogen, poly(3-hydroxybutyrate) and pigment accumulation in threeJournal of applied phycology · 2022Article
- Biotechnological Conversion of Grape Pomace to Poly(3-hydroxybutyrate) by Moderately Thermophilic BacteriumBioengineering (Basel, Switzerland) · 2021Article
- Sea-Ice BacteriaApplied and environmental microbiology · 2021Article
- Anaerobic Sulfur Oxidation Underlies Adaptation of a Chemosynthetic Symbiont to Oxic-Anoxic Interfaces.mSystems · 2021Article
- Article
- Fructose-Based Production of Short-Chain-Length and Medium-Chain-Length Polyhydroxyalkanoate Copolymer by ArcticPolymers · 2021Article
- The First Insight into Polyhydroxyalkanoates Accumulation in Multi-ExtremophilicMicroorganisms · 2021Article
- Assessing Viability and Stress Tolerance of Probiotics-A Review.Frontiers in microbiology · 2021Review
Corrections and comments
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Authors and funding
9 authors at 2 institutions in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Accumulation of polyhydroxybutyrate (PHB) seems to be a common metabolic strategy adopted by many bacteria to cope with cold environments. This work aimed at evaluating and understanding the cryoprotective effect of PHB. At first a monomer of PHB, 3-hydroxybutyrate, was identified as a potent cryoprotectant capable of protecting model enzyme (lipase), yeast (Saccharomyces cerevisiae) and bacterial cells (Cupriavidus necator) against the adverse effects of freezing-thawing cycles. Further, the viability of the frozen-thawed PHB accumulating strain of C. necator was compared to that of the PHB non-accumulating mutant. The presence of PHB granules in cells was revealed to be a significant advantage during freezing. This might be attributed to the higher intracellular level of 3-hydroxybutyrate in PHB accumulating cells (due to the action of parallel PHB synthesis and degradation, the so-called PHB cycle), but the cryoprotective effect of PHB granules seems to be more complex. Since intracellular PHB granules retain highly flexible properties even at extremely low temperatures (observed by cryo-SEM), it can be expected that PHB granules protect cells against injury from extracellular ice. Finally, thermal analysis indicates that PHB-containing cells exhibit a higher rate of transmembrane water transport, which protects cells against the formation of intracellular ice which usually has fatal consequences.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.