ArticlePloS one2016
Transgenic Soybean Production of Bioactive Human Epidermal Growth Factor (EGF).
Article in PloS one, 2016. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 11 papers.
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
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Who cites it
11 citing papers in PubMed, 30 citations in OpenAlex.
- Efficient expression of fusion human epidermal growth factor in tobacco chloroplasts.BMC biotechnology · 2023Article
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- Enzymatic degradation is an effective means to reduce aflatoxin contamination in maize.BMC biotechnology · 2021Article
- Epithelial wound healing in inflammatory bowel diseases: the next therapeutic frontier.Translational research : the journal of laboratory and clinical medicine · 2021Review
- Expression optimization, purification andBiotechnology reports (Amsterdam, Netherlands) · 2020Article
- Epidermal growth factor as a reliable marker of necrotizing enterocolitis in preterm neonates.Clinical and experimental pediatrics · 2020Article
- Shotguns vs Lasers: Identifying barriers and facilitators to scaling-up plant molecular farming for high-value health products.PloS one · 2020Article
- Characterization and functional biology of the soybean aleurone layer.BMC plant biology · 2018Article
- Adrenergic receptor stimulation suppresses oxidative metabolism in isolated rat islets and Min6 cells.Molecular and cellular endocrinology · 2018Article
- Pharmacological activation of epidermal growth factor receptor signaling inhibits colitis-associated cancer in mice.Scientific reports · 2018Article
- Soy-Based Therapeutic Baby Formulas: Testable Hypotheses Regarding the Pros and Cons.Frontiers in nutrition · 2016Article
Corrections and comments
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Authors and funding
8 authors at 2 institutions in 1 country.
Funding
Abstract
Necrotizing enterocolitis (NEC) is a devastating condition of premature infants that results from the gut microbiome invading immature intestinal tissues. This results in a life-threatening disease that is frequently treated with the surgical removal of diseased and dead tissues. Epidermal growth factor (EGF), typically found in bodily fluids, such as amniotic fluid, salvia and mother's breast milk, is an intestinotrophic growth factor and may reduce the onset of NEC in premature infants. We have produced human EGF in soybean seeds to levels biologically relevant and demonstrated its comparable activity to commercially available EGF. Transgenic soybean seeds expressing a seed-specific codon optimized gene encoding of the human EGF protein with an added ER signal tag at the N' terminal were produced. Seven independent lines were grown to homozygous and found to accumulate a range of 6.7 +/- 3.1 to 129.0 +/- 36.7 μg EGF/g of dry soybean seed. Proteomic and immunoblot analysis indicates that the inserted EGF is the same as the human EGF protein. Phosphorylation and immunohistochemical assays on the EGF receptor in HeLa cells indicate the EGF protein produced in soybean seed is bioactive and comparable to commercially available human EGF. This work demonstrates the feasibility of using soybean seeds as a biofactory to produce therapeutic agents in a soymilk delivery platform.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.