ArticleBioMed research international2016
Development of a Rapid Point-of-Use DNA Test for the Screening of Genuity® Roundup Ready 2 Yield® Soybean in Seed Samples.
Article in BioMed research international, 2016. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 14 papers.
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Who cites it
14 citing papers in PubMed.
- Rapid isothermal molecular tests to discriminate between Leishmania braziliensis and Leishmania infantum infections in dogs.Parasites & vectors · 2025Article
- Research Progress of Nucleic Acid Detection Technology for Genetically Modified Maize.International journal of molecular sciences · 2023Review
- Malaria diagnostic update: From conventional to advanced method.Journal of clinical laboratory analysis · 2022Review
- Recombinase Polymerase Amplification Combined with Real-Time Fluorescent Probe forJournal of clinical medicine · 2022Article
- Development of real-time and lateral flow recombinase polymerase amplification assays for rapid detection ofFrontiers in microbiology · 2022Article
- Rapid and direct detection of hepatitis E virus in raw pork livers by recombinase polymerase amplification assays.Frontiers in cellular and infection microbiology · 2022Article
- The Potential Use of Isothermal Amplification Assays for In-Field Diagnostics of Plant Pathogens.Plants (Basel, Switzerland) · 2021Review
- Characterization and a RT-RPA assay for rapid detection of Chilli Veinal mottle virus (ChiVMV) in tobacco.Virology journal · 2020Article
- Article
- Multiplex Detection of Nucleic Acids Using Recombinase Polymerase Amplification and a Molecular Colorimetric 7-Segment Display.ACS omega · 2019Article
- Nucleic Acid Detection of Plant Genes Using CRISPR-Cas13.The CRISPR journal · 2019Article
- Rapid detection of potyviruses from crude plant extracts.Analytical biochemistry · 2018Article
- Recombinase polymerase amplification: Basics, applications and recent advances.Trends in analytical chemistry : TRAC · 2018Review
- Article
Corrections and comments
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Authors and funding
10 authors.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Testing for the presence of genetically modified material in seed samples is of critical importance for all stakeholders in the agricultural industry, including growers, seed manufacturers, and regulatory bodies. While rapid antibody-based testing for the transgenic protein has fulfilled this need in the past, the introduction of new variants of a given transgene demands new diagnostic regimen that allows distinguishing different traits at the nucleic acid level. Although such molecular tests can be performed by PCR in the laboratory, their requirement for expensive equipment and sophisticated operation have prevented its uptake in point-of-use applications. A recently developed isothermal DNA amplification technique, recombinase polymerase amplification (RPA), combines simple sample preparation and amplification work-flow procedures with the use of minimal detection equipment in real time. Here, we report the development of a highly sensitive and specific RPA-based detection system for Genuity Roundup Ready 2 Yield (RR2Y) material in soybean (Glycine max) seed samples and present the results of studies applying the method in both laboratory and field-type settings.
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