ArticleBioMed research international2016
Identification and Validation of Reference Genes for qRT-PCR Studies of Gene Expression in Dioscorea opposita.
Article in BioMed research international, 2016. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 13 papers.
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Who cites it
13 citing papers in PubMed.
- Efficacy of chitinases from mangrove wetland derivedFrontiers in microbiology · 2026Article
- Reference genes selection and validation in yam by real-time quantitative polymerase chain reaction.Scientific reports · 2025Article
- Selection and validation of stable reference genes in potato infected by Pectobacterium atrosepticum using real-time quantitative PCR.Scientific reports · 2025Article
- Systematic identification of reference genes for qRT-PCR ofFrontiers in plant science · 2023Article
- Review
- Integrated mRNA and miRNA transcriptome analysis reveals a regulatory network for tuber expansion in Chinese yam (Dioscorea opposita).BMC genomics · 2020Article
- Transcriptome Analysis Reveals Key Pathways and Hormone Activities Involved in Early Microtuber Formation ofBioMed research international · 2020Article
- Validation of suitable reference genes for quantitative gene expression analysis in Tripterygium wilfordii.Molecular biology reports · 2019Article
- Identification of candidate flowering and sex genes in white Guinea yam (D. rotundata Poir.) by SuperSAGE transcriptome profiling.PloS one · 2019Article
- Assessment of Aedes albopictus reference genes for quantitative PCR at different stages of development.PloS one · 2018Article
- Identification and validation of reference genes for qRT-PCR analysis in mulberry (Morus alba L.).PloS one · 2018Article
- Validation of reference genes for the normalization of the RT-qPCR gene expression of virulence genes of Erwinia amylovora in apple shoots.Scientific reports · 2017Article
- Validation of Suitable Reference Genes for RT-qPCR Data inFrontiers in plant science · 2017Article
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9 authors.
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Abstract
Quantitative real-time polymerase chain reaction (qRT-PCR) is one of the most common methods for gene expression studies. Data normalization based on reference genes is essential for obtaining reliable results for qRT-PCR assays. This study evaluated potential reference genes of Chinese yam (Dioscorea opposita Thunb.), which is an important tuber crop and medicinal plant in East Asia. The expression of ten candidate reference genes across 20 samples from different organs and development stages was assessed. We identified the most stable genes for qRT-PCR studies using combined samples from different organs. Our results also suggest that different suitable reference genes or combinations of reference genes for normalization should be applied according to different organs and developmental stages. To validate the suitability of the reference genes, we evaluated the relative expression of PE2.1 and PE53, which are two genes that may be associated with microtuber formation. Our results provide the foundation for reference gene(s) selection in D. opposita and will contribute toward more accurate gene analysis studies of the genus Dioscorea.
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