Evidence map›Paper›PMID 27313078›Full record

ArticleFEBS letters2016

Effect of chaperone-adhesin complex on plug release by the PapC usher.

Thieng Pham, Glenn T Werneburg, Nadine S Henderson, David G Thanassi, Anne H Delcour

Abstract readLetter
PubMed Full text at PMC Publisher
In one paragraph

Article in FEBS letters, 2016. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 1 paper.

0numbers the graph read from it
0cells of the map it votes in
1citing papers in PubMed
0.6field-weighted citation impact, top 28% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

1 citing paper in PubMed, 3 citations in OpenAlex.

  1. Article
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

5 authors at 2 institutions in 1 country.

Thieng PhamDepartment of Biology and Biochemistry, University of Houston, TX, USA.
Glenn T WerneburgDepartment of Molecular Genetics and Microbiology, Center for Infectious Diseases, Stony Brook University, NY, USA.
Nadine S HendersonDepartment of Molecular Genetics and Microbiology, Center for Infectious Diseases, Stony Brook University, NY, USA.
David G ThanassiDepartment of Molecular Genetics and Microbiology, Center for Infectious Diseases, Stony Brook University, NY, USA.
Anne H DelcourDepartment of Biology and Biochemistry, University of Houston, TX, USA.
Stony Brook University · USUniversity of Houston · US

Funding

Mechanism of the Usher in Assembly and Secretion of PiliR01GM062987 · NIGMS · STATE UNIVERSITY NEW YORK STONY BROOK · PI David G Thanassi · 2001 to 2026
$7.7M
Catalytic mechanism of the usher in pilus biogenesis by uropathogenic E. coliF30AI112252 · NIAID · STATE UNIVERSITY NEW YORK STONY BROOK · PI WERNEBURG, GLENN THOMAS · 2015 to 2018
$162k
NIAID NIH HHS F30 AI112252NIGMS NIH HHS R01 GM062987
6 · The paper itself

Abstract

The P pilus of uropathogenic Escherichia coli is a multisubunit fiber assembled at the outer membrane in a defined sequence by a chaperone/usher secretion system, comprising a periplasmic chaperone and a beta-barrel outer membrane protein, the PapC usher. To gain insight into the pilus biogenesis mechanism, we used patch clamp electrophysiology to investigate the effect of the initiating adhesin subunit, as it is delivered to PapC in a complex with the chaperone. We show that the chaperone-adhesin complex facilitates opening of the PapC pore and appears to engage within the PapC lumen, in agreement with prior biochemical and structural data.

Indexed as

Adhesins, BacterialEscherichia coli ProteinsMolecular ChaperonesMultiprotein ComplexesPeriplasmic ProteinsPorinsUropathogenic Escherichia coliAdhesins, BacterialatpG protein, E coliEscherichia coli ProteinsMolecular ChaperonesMultiprotein ComplexesPeriplasmic ProteinsPorinsE. coliouter membranepatch clampP pilussecretion

Identifiers

PMID27313078
PMCPMC4963266
OpenAlexW2425698253

What OpenQuestion holds

Textmetadata
LicenceTDM
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.