ArticleScientific reports2016
Light-sheet microscopy imaging of a whole cleared rat brain with Thy1-GFP transgene.
Article in Scientific reports, 2016. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 47 papers.
What it found
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The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
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Who cites it
47 citing papers in PubMed, 104 citations in OpenAlex.
- A uniform tissue-clearing framework and mesoSPIM-ultra enable cm-scale single-neuron tracing.bioRxiv : the preprint server for biology · 2026Article
- Isotropic, aberration-corrected light sheet microscopy for rapid high-resolution imaging of cleared tissue.Nature biotechnology · 2025Article
- Tauroursodeoxycholic acid-induced increase in ectopic muscle mineralization occurs exclusively in dystrophic muscles and is independent of endoplasmic reticulum stress.Scientific reports · 2025Article
- In Toto Adipocytes Analysis Using Hydrophilic Tissue Clearing, Light Sheet Microscopy, and Deep Learning-Based Image Processing.Biology of the cell · 2025Article
- A 3D spheroid model for assessing nanocarrier-based drug delivery to solid tumors.npj biomedical innovations · 2025Article
- Frontiers in artificial intelligence-directed light-sheet microscopy for uncovering biological phenomena and multi-organ imaging.View (Beijing, China) · 2024Article
- descSPIM: an affordable and easy-to-build light-sheet microscope optimized for tissue clearing techniques.Nature communications · 2024Article
- Interferometric modulation for generating extended light sheet: improving field of view.Journal of biomedical optics · 2024Article
- Multiscale elasticity mapping of biological samples in 3D at optical resolution.Acta biomaterialia · 2024Article
- Multi-resolution X-ray phase-contrast and dark-field tomography of human cerebellum with near-field speckles.Biomedical optics express · 2024Article
- T-CLEARE: a pilot community-driven tissue clearing protocol repository.Frontiers in bioengineering and biotechnology · 2024Article
- Princeton RAtlas: A Common Coordinate Framework for Fully cleared, WholeBio-protocol · 2023Article
- High-resolution visualization of pial surface vessels by flattened whole mount staining.iScience · 2023Article
- Whole-brain tracking of cocaine and sugar rewards processing.Translational psychiatry · 2023Article
- Comparison of Muscle Regeneration after BMSC-Conditioned Medium, Syngeneic, or Allogeneic BMSC Injection.Cells · 2022Article
- Standard metadata for 3D microscopy.Scientific data · 2022Article
- Automated computation of nerve fibre inclinations from 3D polarised light imaging measurements of brain tissue.Scientific reports · 2022Article
- Mesoscale microscopy and image analysis tools for understanding the brain.Progress in biophysics and molecular biology · 2022Review
- Opposing roles for striatonigral and striatopallidal neurons in dorsolateral striatum in consolidating new instrumental actions.Nature communications · 2021Article
- miniSPIM-A Miniaturized Light-Sheet Microscope.ACS sensors · 2021Article
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
11 authors at 2 institutions in 2 countries.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Whole-brain imaging with light-sheet fluorescence microscopy and optically cleared tissue is a new, rapidly developing research field. Whereas successful attempts to clear and image mouse brain have been reported, a similar result for rats has proven difficult to achieve. Herein, we report on creating novel transgenic rat harboring fluorescent reporter GFP under control of neuronal gene promoter. We then present data on clearing the rat brain, showing that FluoClearBABB was found superior over passive CLARITY and CUBIC methods. Finally, we demonstrate efficient imaging of the rat brain using light-sheet fluorescence microscopy.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.