ArticleBiotechnology journal2016
Cell-to-cell heterogeneity emerges as consequence of metabolic cooperation in a synthetic yeast community.
Article in Biotechnology journal, 2016. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 19 papers.
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Who cites it
19 citing papers in PubMed.
- Emergent phenotypic heterogeneity in synthetic cross-feeding consortia driven by heterogeneous metabolite distribution.National science review · 2026Article
- Scale-down bioreactors-comparative analysis of configurations.Bioprocess and biosystems engineering · 2025Review
- A dual reporter system for intracellular and extracellular amino acid sensing in budding yeast.Molecular biology of the cell · 2025Article
- Metabolic heterogeneity and cross-feeding within isogenic yeast populations captured by DILAC.Nature microbiology · 2023Article
- Emergence of Phenotypically Distinct Subpopulations Is a Factor in Adaptation of Recombinant Saccharomyces cerevisiae under Glucose-Limited Conditions.Applied and environmental microbiology · 2022Article
- Microbial communities form rich extracellular metabolomes that foster metabolic interactions and promote drug tolerance.Nature microbiology · 2022Article
- Monitoring single-cell dynamics of entry into quiescence during an unperturbed life cycle.eLife · 2021Article
- Article
- Aspects of Multicellularity inGenes · 2020Review
- A new protocol for single-cell RNA-seq reveals stochastic gene expression during lag phase in budding yeast.eLife · 2020Article
- Single-Cell Technologies to Understand the Mechanisms of Cellular Adaptation in Chemostats.Frontiers in bioengineering and biotechnology · 2020Article
- Article
- Interrogating metabolism as an electron flow system.Current opinion in systems biology · 2019Review
- Self-Establishing Communities: A Yeast Model to Study the Physiological Impact of Metabolic Cooperation in Eukaryotic Cells.Methods in molecular biology (Clifton, N.J.) · 2019Article
- A minimal "push-pull" bistability model explains oscillations between quiescent and proliferative cell states.Molecular biology of the cell · 2018Article
- Article
- Impact of spatial organization on a novel auxotrophic interaction among soil microbes.The ISME journal · 2018Article
- Saccharomyces cerevisiae metabolism in ecological context.FEMS yeast research · 2016Review
- Article
Corrections and comments
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Authors and funding
3 authors.
Funding
Abstract
Cells that grow together respond heterogeneously to stress even when they are genetically similar. Metabolism, a key determinant of cellular stress tolerance, may be one source of this phenotypic heterogeneity, however, this relationship is largely unclear. We used self-establishing metabolically cooperating (SeMeCo) yeast communities, in which metabolic cooperation can be followed on the basis of genotype, as a model to dissect the role of metabolic cooperation in single-cell heterogeneity. Cells within SeMeCo communities showed to be highly heterogeneous in their stress tolerance, while the survival of each cell under heat or oxidative stress, was strongly determined by its metabolic specialization. This heterogeneity emerged for all metabolite exchange interactions studied (histidine, leucine, uracil, and methionine) as well as oxidant (H2 O2 , diamide) and heat stress treatments. In contrast, the SeMeCo community collectively showed to be similarly tolerant to stress as wild-type populations. Moreover, stress heterogeneity did not establish as sole consequence of metabolic genotype (auxotrophic background) of the single cell, but was observed only for cells that cooperated according to their metabolic capacity. We therefore conclude that phenotypic heterogeneity and cell to cell differences in stress tolerance are emergent properties when cells cooperate in metabolism.
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