Evidence map›Paper›PMID 27310614›Full record

ArticleRNA biology2016

PacBio full-length transcriptome profiling of insect mitochondrial gene expression.

Shan Gao, Yipeng Ren, Yu Sun, Zhenfeng Wu, Jishou Ruan, Bingjun He, Tao Zhang, Xin Yu, Xiaoxuan Tian, Wenjun Bu

Erratum issuedAbstract read
In one paragraph

Article in RNA biology, 2016. The graph could read no effect estimate from its abstract, so it casts no vote on the map. An erratum has been issued. Cited by 29 papers.

0numbers the graph read from it
0cells of the map it votes in
29citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

29 citing papers in PubMed.

  1. Review
  2. Unravelling the complete mitochondrial genomes ofFrontiers in insect science · 2025
    Article
  3. Article
  4. Article
  5. Article
  6. The genome and transcriptome of the snailbioRxiv : the preprint server for biology · 2023
    Article
  7. Article
  8. Article
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  10. Article
  11. Article
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  15. Review
  16. Article
  17. Article
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4 · The record

Corrections and comments

5 · Who and what money

Authors and funding

10 authors.

Shan Gaoa College of Life Sciences, Nankai University , Tianjin, Tianjin , P.R. China.
Yipeng Rena College of Life Sciences, Nankai University , Tianjin, Tianjin , P.R. China.
Yu Suna College of Life Sciences, Nankai University , Tianjin, Tianjin , P.R. China.
Zhenfeng Wub School of Mathematical Sciences, Nankai University , Tianjin , P.R.China.
Jishou Ruanb School of Mathematical Sciences, Nankai University , Tianjin , P.R.China.
Bingjun Hea College of Life Sciences, Nankai University , Tianjin, Tianjin , P.R. China.
Tao Zhanga College of Life Sciences, Nankai University , Tianjin, Tianjin , P.R. China.
Xin Yua College of Life Sciences, Nankai University , Tianjin, Tianjin , P.R. China.
Xiaoxuan Tianc Tianjin State Key Laboratory of Modern Chinese Medicine, Tianjin University of Traditional Chinese Medicine , Tianjin , P.R. China.
Wenjun Bua College of Life Sciences, Nankai University , Tianjin, Tianjin , P.R. China.

Funding

No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

In this study, we sequenced the first full-length insect transcriptome using the Erthesina fullo Thunberg based on the PacBio platform. We constructed the first quantitative transcription map of animal mitochondrial genomes and built a straightforward and concise methodology to investigate mitochondrial gene transcription, RNA processing, mRNA maturation and several other related topics. Most of the results were consistent with the previous studies, while to the best of our knowledge some findings were reported for the first time in this study. The new findings included the high levels of mitochondrial gene expression, the 3' polyadenylation and possible 5' m(7)G caps of rRNAs, the isoform diversity of 12S rRNA, the polycistronic transcripts and natural antisense transcripts of mitochondrial genes et al. These findings could challenge and enrich fundamental concepts of mitochondrial gene transcription and RNA processing, particularly of the rRNA primary (sequence) structure. The methodology constructed in this study can also be used to study gene expression or RNA processing of nuclear genomes.

Indexed as

Gene Expression ProfilingGenes, InsectGenes, MitochondrialTranscriptomeAnimalsComputational BiologyGene Expression RegulationGene OrderGenome, MitochondrialHigh-Throughput Nucleotide SequencingInsectaRNA, AntisenseRNA IsoformsRNA, MessengerRNA PrecursorsRNA Processing, Post-TranscriptionalRNA, AntisenseRNA IsoformsRNA, MessengerRNA PrecursorsRNA, RibosomalFull-length transcriptomegene transcriptionmitochondrial genesPacBioRNA processing

Identifiers

PMID27310614
PMCPMC5014001

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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.