ArticleTissue engineering. Part C, Methods2016
Comparative Decellularization and Recellularization of Wild-Type and Alpha 1,3 Galactosyltransferase Knockout Pig Lungs: A Model for Ex Vivo Xenogeneic Lung Bioengineering and Transplantation.
Article in Tissue engineering. Part C, Methods, 2016. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 22 papers.
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Who cites it
22 citing papers in PubMed.
- Genetically modified pigs with α1,3-galactosyltransferase knockout and beyond: a comprehensive review of xenotransplantation strategies.Frontiers in immunology · 2025Review
- Designing injectable dermal matrix hydrogel combined with silver nanoparticles for methicillin-resistant Staphylococcus aureus infected wounds healing.Nano convergence · 2024Article
- Decellularized Matrices for the Treatment of Tissue Defects: from Matrix Origin to Immunological Mechanisms.Biomolecules & therapeutics · 2024Review
- Two Decades of Advances and Limitations in Organ Recellularization.Current issues in molecular biology · 2024Review
- Unlocking the Promise of Decellularized Pancreatic Tissue: A Novel Approach to Support Angiogenesis in Engineered Tissue.Bioengineering (Basel, Switzerland) · 2024Article
- Evaluation of a decellularized bronchial patch transplant in a porcine model.Scientific reports · 2023Article
- Engineering Dynamic 3D Models of Lung.Advances in experimental medicine and biology · 2023Article
- What is the need and why is it time for innovative models for understanding lung repair and regeneration?Frontiers in pharmacology · 2023Review
- Advances in lung bioengineering: Where we are, where we need to go, and how to get there.Frontiers in transplantation · 2023Review
- Decellularization and Recellularization Methods for Avian Lungs: An Alternative Approach for Use in Pulmonary Therapeutics.Methods in molecular biology (Clifton, N.J.) · 2022Article
- Biomanufacturing of Axon-Based Tissue Engineered Nerve Grafts Using Porcine GalSafe Neurons.Tissue engineering. Part A · 2021Article
- Engineering a 3D Vascularized Adipose Tissue Construct Using a Decellularized Lung Matrix.Biomimetics (Basel, Switzerland) · 2021Article
- Sterilization and disinfection methods for decellularized matrix materials: Review, consideration and proposal.Bioactive materials · 2021Review
- Bioengineering the Blood-gas Barrier.Comprehensive Physiology · 2020Review
- Functional role of glycosaminoglycans in decellularized lung extracellular matrix.Acta biomaterialia · 2020Article
- Article
- Lung bioengineering: advances and challenges in lung decellularization and recellularization.Current opinion in organ transplantation · 2018Review
- Cell-based Therapy for Chronic Obstructive Pulmonary Disease. Rebuilding the Lung.Annals of the American Thoracic Society · 2018Review
- Development of a Cytocompatible Scaffold from Pig Immature Testicular Tissue Allowing Human Sertoli Cell Attachment, Proliferation and Functionality.International journal of molecular sciences · 2018Article
- Avian lungs: A novel scaffold for lung bioengineering.PloS one · 2018Article
Corrections and comments
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Authors and funding
19 authors.
Funding
Abstract
backgroundA novel potential approach for lung transplantation could be to utilize xenogeneic decellularized pig lung scaffolds that are recellularized with human lung cells. However, pig tissues express several immunogenic proteins, notably galactosylated cell surface glycoproteins resulting from alpha 1,3 galactosyltransferase (α-gal) activity, that could conceivably prevent effective use. Use of lungs from α-gal knock out (α-gal KO) pigs presents a potential alternative and thus comparative de- and recellularization of wild-type and α-gal KO pig lungs was assessed.
methodsDecellularized lungs were compared by histologic, immunohistochemical, and mass spectrometric techniques. Recellularization was assessed following compartmental inoculation of human lung bronchial epithelial cells, human lung fibroblasts, human bone marrow-derived mesenchymal stromal cells (all via airway inoculation), and human pulmonary vascular endothelial cells (CBF) (vascular inoculation).
resultsNo obvious differences in histologic structure was observed but an approximate 25% difference in retention of residual proteins was determined between decellularized wild-type and α-gal KO pig lungs, including retention of α-galactosylated epitopes in acellular wild-type pig lungs. However, robust initial recellularization and subsequent growth and proliferation was observed for all cell types with no obvious differences between cells seeded into wild-type versus α-gal KO lungs.
conclusionThese proof of concept studies demonstrate that decellularized wild-type and α-gal KO pig lungs can be comparably decellularized and comparably support initial growth of human lung cells, despite some differences in retained proteins. α-Gal KO pig lungs are a suitable platform for further studies of xenogeneic lung regeneration.
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