ArticleNature protocols2016
Electrochemical detection of nucleic acids, proteins, small molecules and cells using a DNA-nanostructure-based universal biosensing platform.
Article in Nature protocols, 2016. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 89 papers.
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Who cites it
89 citing papers in PubMed, 383 citations in OpenAlex.
- Tetrahedral framework nucleic acids: Nanokeys unlocking a new era of precision biomedicine.Journal of advanced research · 2026Review
- CRISPR/Cas13a: Compensatory Target Activation Mechanism.Advanced science (Weinheim, Baden-Wurttemberg, Germany) · 2026Article
- Biomolecule Detection Methods Based on Nanoparticle Approaches.ACS omega · 2026Review
- DNA framework-based molecular transformer for logic-driven precision diagnostics.Science advances · 2026Article
- Horseradish peroxidase as an electrochemical reporter protein for cell-free biosensors.Synthetic biology (Oxford, England) · 2026Article
- Highly Ordered DNA Framework Interface Enables Efficient Enzymatic Oligonucleotide Synthesis.Advanced science (Weinheim, Baden-Wurttemberg, Germany) · 2025Article
- Role of the real first interface in regulating ionic signal of nanochannels.Nature communications · 2025Article
- A highly sensitive label-free aptamer sensor for timely detection of human serum albumin.RSC advances · 2025Article
- Tetrahedral-modified magnetic nanorobotic probe for enhanced imaging of cancer-related miRNA.Microsystems & nanoengineering · 2025Article
- Biosensors, Artificial Intelligence Biosensors, False Results and Novel Future Perspectives.Diagnostics (Basel, Switzerland) · 2025Review
- Scalable electrochemical system for rapid on-site detection of food allergens.Biosensors & bioelectronics · 2025Article
- Spontaneous Charging from Sliding Water Drops Determines the Interfacial Deposition of Charged Solutes.Advanced materials (Deerfield Beach, Fla.) · 2025Article
- Detection of Exosomal microRNA from Malignant Lung Cells Using a Surface Plasmon Resonance Imaging Biosensor.Biosensors · 2025Article
- Review
- Article
- Immunochromatographic Strip Based on Tetrahedral DNA Immunoprobe for the Detection of Aflatoxin BFoods (Basel, Switzerland) · 2024Article
- Functionalized tetrahedral DNA frameworks for the capture of circulating tumor cells.Nature protocols · 2024Review
- Long-term monitoring of ultratrace nucleic acids using tetrahedral nanostructure-based NgAgo on wearable microneedles.Nature communications · 2024Article
- Polydopamine-assisted aptamer-carrying tetrahedral DNA microelectrode sensor for ultrasensitive electrochemical detection of exosomes.Journal of nanobiotechnology · 2024Article
- An impedance labeling free electrochemical aptamer sensor based on tetrahedral DNA nanostructures for doxorubicin determination.Mikrochimica acta · 2024Article
29 more citing papers are in PubMed but not listed here.
Corrections and comments
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Authors and funding
8 authors at 5 institutions in 4 countries.
Funding
No grant is acknowledged in the PubMed record.
Abstract
The occurrence and prognosis of many complex diseases, such as cancers, is associated with the variation of various molecules, including DNA at the genetic level, RNA at the regulatory level, proteins at the functional level and small molecules at the metabolic level (defined collectively as multilevel molecules). Thus it is highly desirable to develop a single platform for detecting multilevel biomarkers for early-stage diagnosis. Here we report a protocol on DNA-nanostructure-based programmable engineering of the biomolecular recognition interface, which provides a universal electrochemical biosensing platform for the ultrasensitive detection of nucleic acids (DNA/RNA), proteins, small molecules and whole cells. The protocol starts with the synthesis of a series of differentially sized, self-assembled tetrahedral DNA nanostructures (TDNs) with site-specifically modified thiol groups that can be readily anchored on the surface of a gold electrode with high reproducibility. By exploiting the rigid structure, nanoscale addressability and versatile functionality of TDNs, one can tailor the type of biomolecular probes appended on individual TDNs for the detection of specific molecules of interest. Target binding occurring on the gold surface patterned with TDNs is quantitatively translated into electrochemical signals via a coupled enzyme-based catalytic process. This uses a sandwich assay strategy in which biotinylated reporter probes recognize TDN-bound target biomolecules, which then allow binding of horseradish-peroxidase-conjugated avidin (avidin-HRP). Hydrogen peroxide (H2O2) is then reduced by avidin-HRP in the presence of TMB (3,3',5,5'-tetramethylbenzidine) to generate a quantitative electrochemical signal. The time range for the entire protocol is ∼1 d, whereas the detection process takes ∼30 min to 3 h.
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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.