Evidence map›Paper›PMID 27309940›Full record

ArticleEndocrinology2016

Conditional Ablation of Progesterone Receptor Membrane Component 1 Results in Subfertility in the Female and Development of Endometrial Cysts.

Melissa L McCallum, Cindy A Pru, Yuichi Niikura, Siu-Pok Yee, John P Lydon, John J Peluso, James K Pru

Open access · bronzeAbstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in Endocrinology, 2016. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 33 papers.

0numbers the graph read from it
0cells of the map it votes in
33citing papers in PubMed
8.4field-weighted citation impact, top 3% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

33 citing papers in PubMed, 61 citations in OpenAlex.

  1. Article
  2. UterineCancers · 2025
    Article
  3. Review
  4. Article
  5. Review
  6. Article
  7. Cells · 2023
    Article
  8. PGRMC1: An enigmatic heme-binding protein.Pharmacology & therapeutics · 2023
    Review
  9. Article
  10. Review
  11. Review
  12. Review
  13. Article
  14. Review
  15. Article
  16. Review
  17. Article
  18. Article
  19. Article
  20. Article
4 · The record

Corrections and comments

5 · Who and what money

Authors and funding

7 authors at 3 institutions in 1 country.

Melissa L McCallumDepartment of Animal Sciences (M.L.M., C.A.P., Y.N., J.K.P.), Center for Reproductive Biology, Washington State University, Pullman, Washington 99164; Departments of Cell Biology and Obstetrics and Gynecology (S.P.Y., J.J.P.), University of Connecticut Health Center, Farmington, Connecticut 06030; and Department of Molecular and Cellular Biology (J.P.L.), Baylor College of Medicine, Houston, Texas 77030.
Cindy A PruDepartment of Animal Sciences (M.L.M., C.A.P., Y.N., J.K.P.), Center for Reproductive Biology, Washington State University, Pullman, Washington 99164; Departments of Cell Biology and Obstetrics and Gynecology (S.P.Y., J.J.P.), University of Connecticut Health Center, Farmington, Connecticut 06030; and Department of Molecular and Cellular Biology (J.P.L.), Baylor College of Medicine, Houston, Texas 77030.
Yuichi NiikuraDepartment of Animal Sciences (M.L.M., C.A.P., Y.N., J.K.P.), Center for Reproductive Biology, Washington State University, Pullman, Washington 99164; Departments of Cell Biology and Obstetrics and Gynecology (S.P.Y., J.J.P.), University of Connecticut Health Center, Farmington, Connecticut 06030; and Department of Molecular and Cellular Biology (J.P.L.), Baylor College of Medicine, Houston, Texas 77030.
Siu-Pok YeeDepartment of Animal Sciences (M.L.M., C.A.P., Y.N., J.K.P.), Center for Reproductive Biology, Washington State University, Pullman, Washington 99164; Departments of Cell Biology and Obstetrics and Gynecology (S.P.Y., J.J.P.), University of Connecticut Health Center, Farmington, Connecticut 06030; and Department of Molecular and Cellular Biology (J.P.L.), Baylor College of Medicine, Houston, Texas 77030.
John P LydonDepartment of Animal Sciences (M.L.M., C.A.P., Y.N., J.K.P.), Center for Reproductive Biology, Washington State University, Pullman, Washington 99164; Departments of Cell Biology and Obstetrics and Gynecology (S.P.Y., J.J.P.), University of Connecticut Health Center, Farmington, Connecticut 06030; and Department of Molecular and Cellular Biology (J.P.L.), Baylor College of Medicine, Houston, Texas 77030.
John J PelusoDepartment of Animal Sciences (M.L.M., C.A.P., Y.N., J.K.P.), Center for Reproductive Biology, Washington State University, Pullman, Washington 99164; Departments of Cell Biology and Obstetrics and Gynecology (S.P.Y., J.J.P.), University of Connecticut Health Center, Farmington, Connecticut 06030; and Department of Molecular and Cellular Biology (J.P.L.), Baylor College of Medicine, Houston, Texas 77030.
James K PruDepartment of Animal Sciences (M.L.M., C.A.P., Y.N., J.K.P.), Center for Reproductive Biology, Washington State University, Pullman, Washington 99164; Departments of Cell Biology and Obstetrics and Gynecology (S.P.Y., J.J.P.), University of Connecticut Health Center, Farmington, Connecticut 06030; and Department of Molecular and Cellular Biology (J.P.L.), Baylor College of Medicine, Houston, Texas 77030.
Washington State University · USUConn Health · USBaylor College of Medicine · US

Funding

Testosterone-GnRH Frequency and the Evolution of PCOS in AdolescenceP50HD028934 · NICHD · UNIVERSITY OF VIRGINIA · PI HAISENLEDER, DANIEL J. · 2014 to 2018
$8.6M
Mechanisms of PGRMC2 action in female reproductionR21OD016564 · OD · WASHINGTON STATE UNIVERSITY · PI PRU, JAMES K · 2014 to 2015
$432k
PGRMC1 function in female reproductive physiologyR21RR030264 · NCRR · UNIVERSITY OF CONNECTICUT SCH OF MED/DNT · PI PELUSO, JOHN J · 2010 to 2011
$427k
NCRR NIH HHS R21 RR030264NICHD NIH HHS P50 HD028934NIH HHS R21 OD016564
6 · The paper itself

Abstract

Progesterone (P4) is essential for female fertility. The objective of this study was to evaluate the functional requirement of the nonclassical P4 receptor (PGR), PGR membrane component 1, in regulating female fertility. To achieve this goal, the Pgrmc1 gene was floxed by insertion of loxP sites on each side of exon 2. Pgrmc1 floxed (Pgrmc1(fl/fl)) mice were crossed with Pgr(cre) or Amhr2(cre) mice to delete Pgrmc1 (Pgrmc1(d/d)) from the female reproductive tract. A 6-month breeding trial revealed that conditional ablation of Pgrmc1 with Pgr(cre/+) mice resulted in a 40% reduction (P = .0002) in the number of pups/litter. Neither the capacity to ovulate in response to gonadotropin treatment nor the expression of PGR and the estrogen receptor was altered in the uteri of Pgrmc1(d/d) mice compared with Pgrmc1(fl/fl) control mice. Although conditional ablation of Pgrmc1 from mesenchymal tissue using Amhr2(cre/+) mice did not reduce the number of pups/litter, the total number of litters born in the 6-month breeding trial was significantly decreased (P = .041). In addition to subfertility, conditional ablation of Pgrmc1 using either Amhr2(cre/+) or Pgr(cre/+) mice resulted in the development of endometrial cysts starting around 4 months of age. Interestingly, pregnancy attenuated the formation of these uterine cysts. These new findings demonstrate that PGR membrane component 1 plays an important role in female fertility and uterine tissue homeostasis.

Indexed as

FertilityAnimalsCystsEndometriumEstrogen Receptor alphaFemaleGene SilencingMembrane ProteinsMice, Inbred C57BLRandom AllocationReceptors, ProgesteroneEstrogen Receptor alphaMembrane ProteinsPGRMC1 protein, mouseReceptors, Progesterone

Identifiers

PMID27309940
PMCPMC5007897
OpenAlexW2443102477

What OpenQuestion holds

Textmetadata
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.