Evidence map›Paper›PMID 27308279›Full record

ArticleJournal of arthropod-borne diseases2016

Evaluation of Isotope (32)P Method to Mark Culex pipiens (Diptera: Culicidae) in a Laboratory.

Chongxing Zhang, Guihong Shi, Yuqiang Zhao, Dongmei Yan, Huaiju Li, Hongmei Liu, Itsanun Wiwatanaratanabutr, Maoqing Gong

Abstract read
PubMed Full text at PMC
In one paragraph

Article in Journal of arthropod-borne diseases, 2016. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 1 paper.

0numbers the graph read from it
0cells of the map it votes in
1citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

1 citing paper in PubMed.

  1. Article
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

8 authors.

Chongxing ZhangShandong Institute of Parasitic Diseases, Shandong Academy of Medical Sciences, Jining, Shandong, People's Republic of China; Collaborative Innovation Center for the Origin and Control of Emerging Infectious Diseases, Taishan Medical University, Taian, Shandong 271000, China.
Guihong ShiShandong Institute of Parasitic Diseases, Shandong Academy of Medical Sciences, Jining, Shandong, People's Republic of China.
Yuqiang ZhaoShandong Institute of Parasitic Diseases, Shandong Academy of Medical Sciences, Jining, Shandong, People's Republic of China.
Dongmei YanShandong Institute of Parasitic Diseases, Shandong Academy of Medical Sciences, Jining, Shandong, People's Republic of China.
Huaiju LiShandong Institute of Parasitic Diseases, Shandong Academy of Medical Sciences, Jining, Shandong, People's Republic of China.
Hongmei LiuShandong Institute of Parasitic Diseases, Shandong Academy of Medical Sciences, Jining, Shandong, People's Republic of China.
Itsanun WiwatanaratanabutrDepartment of Plant Production Technology, Faculty of Agricultural Technology, King Mongkut's Institute of Technology Ladkrabang, Bangkok 10520, Thailand.
Maoqing GongShandong Institute of Parasitic Diseases, Shandong Academy of Medical Sciences, Jining, Shandong, People's Republic of China.

Funding

No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

backgroundThe aim of the current study was to develop a marking technique as an internal marker to mark post blood meal mosquitoes by using stable phosphate isotope (32)P and determine the optimal concentration of it.

methodsAn isotonic physiological saline solution, containing different concentration of radioactive isotope (32)P-labeled disodium phosphate (Na2H(32)PO4) was injected into rabbits via the jugular vein in the laboratory. Emerged Cx. pipiens were marked after feeding on rabbit. At the same time, the labeled conditions of emerged Cx. pipiens were also measured by placing feces of No. 6 rabbit into containers with mosquito larvae and pupae inside.

resultsAccording to the label condition of Cx. pipiens after taking blood and the effect of different dosage Na2H(32)PO4 on rabbit health, the optimal concentration of radioactive isotope was determined, that is, 0.1211 mCi/kg. By placing feces of No. 6 rabbit into containers with mosquito larvae and pupae inside, the emerged mosquitoes were also labeled. Therefore, feeding mosquitoes on the animal injected with radioactive Na2H(32)PO4 was more practical for detecting and tracing mosquitoes.

conclusionThe method was less time-consuming, more sensitive and safer. This marking method will facilitate post-bloodmeal studies of mosquitoes and other blood-sucking insects.

Indexed as

Culex pipiensMarkRabbitRadioactive isotope

Identifiers

PMID27308279
PMCPMC4906760

What OpenQuestion holds

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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.