ArticleAdvanced biomedical research2016
Induction of chondrogenic differentiation of human adipose-derived stem cells by low frequency electric field.
Article in Advanced biomedical research, 2016. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 22 papers.
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Who cites it
22 citing papers in PubMed, 35 citations in OpenAlex.
- Overcoming challenges in cartilage regeneration: The role of chondrogenic inducers.Bioengineering & translational medicine · 2026Review
- Direct Scaffold-Coupled Electrical Stimulation of Chondrogenic Progenitor Cells through Graphene Foam Bioscaffolds to Control the Mechanical Properties of Graphene Foam-Cell Composites.ACS applied materials & interfaces · 2025Article
- Use of adipose-derived stem cells on decellularized bladder scaffolds for functional bladder mucosa regeneration.Stem cells translational medicine · 2025Article
- Review
- Electrical stimulation for cartilage tissue engineering - A critical review from an engineer's perspective.Heliyon · 2024Review
- Electrical Stimulation of Mesenchymal Stem Cells as a Tool for Proliferation and Differentiation in Cartilage Tissue Engineering: A Scaffold-Based Approach.Bioengineering (Basel, Switzerland) · 2024Article
- Effect of Electrical Stimulation on PC12 Cells Cultured in Different Hydrogels: Basis for the Development of Biomaterials in Peripheral Nerve Tissue Engineering.Pharmaceutics · 2023Article
- Electrical Stimulation in Cartilage Tissue Engineering.Bioengineering (Basel, Switzerland) · 2023Review
- Effects of Electrical Stimulation on Articular Cartilage Regeneration with a Focus on Piezoelectric Biomaterials for Articular Cartilage Tissue Repair and Engineering.International journal of molecular sciences · 2023Review
- Nanosecond pulsed electric fields prime mesenchymal stem cells to peptide ghrelin and enhance chondrogenesis and osteochondral defect repair in vivo.Science China. Life sciences · 2022Article
- Targeted Activation of G-Protein Coupled Receptor-Mediated CaTissue engineering. Part A · 2022Article
- Electric field stimulation for tissue engineering applications.BMC biomedical engineering · 2021Review
- Recent Developed Strategies for Enhancing Chondrogenic Differentiation of MSC: Impact on MSC-Based Therapy for Cartilage Regeneration.Stem cells international · 2021Review
- Establishment of a New Device for Electrical Stimulation of Non-Degenerative Cartilage Cells In Vitro.International journal of molecular sciences · 2021Article
- Continuous Electrical Stimulation Affects Initial Growth and Proliferation of Adipose-Derived Stem Cells.Biomedicines · 2020Article
- Electrical stimulation in bone tissue engineering treatments.European journal of trauma and emergency surgery : official publication of the European Trauma Society · 2020Review
- Changing Expression Profiles and Inclination to Competing Endogenous RNA Networks on MAPK Signaling Pathways of Human Adipose-Derived Stem Cells in a Direct Current Electric Field.BioMed research international · 2020Article
- Numerical Simulation of Electroactive Hydrogels for Cartilage-Tissue Engineering.Materials (Basel, Switzerland) · 2019Article
- Nanosecond pulsed electric fields enhanced chondrogenic potential of mesenchymal stem cells via JNK/CREB-STAT3 signaling pathway.Stem cell research & therapy · 2019Article
- Electrical stimulation as a novel tool for regulating cell behavior in tissue engineering.Biomaterials research · 2019Review
Corrections and comments
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Authors and funding
6 authors at 2 institutions in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
backgroundSince when the cartilage damage (e.g., with the osteoarthritis) it could not be repaired in the body, hence for its reconstruction needs cell therapy. For this purpose, adipose-derived stem cells (ADSCs) is one of the best cell sources because by the tissue engineering techniques it can be differentiated into chondrocytes. Chemical and physical inducers is required order to stem cells to chondrocytes differentiating. We have decided to define the role of electric field (EF) in inducing chondrogenesis process. MATERIALS AND
methodsA low frequency EF applied the ADSCs as a physical inducer for chondrogenesis in a 3D micromass culture system which ADSCs were extracted from subcutaneous abdominal adipose tissue. Also enzyme-linked immunosorbent assay, methyl thiazolyl tetrazolium, real time polymerase chain reaction and flowcytometry techniques were used for this study.
resultsWe found that the 20 minutes application of 1 kHz, 20 mv/cm EF leads to chondrogenesis in ADSCs. Although our results suggest that application of physical (EF) and chemical (transforming growth factor-β3) inducers at the same time, have best results in expression of collagen type II and SOX9 genes. It is also seen EF makes significant decreased expression of collagens type I and X genes.
conclusionThe low frequency EF can be a good motivator to promote chondrogenic differentiation of human ADSCs.
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