ArticleProceedings of the National Academy of Sciences of the United States of America2016
Identification of shared TCR sequences from T cells in human breast cancer using emulsion RT-PCR.
Article in Proceedings of the National Academy of Sciences of the United States of America, 2016. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 38 papers.
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38 citing papers in PubMed, 57 citations in OpenAlex.
- T cell repertoire in peripheral blood as a potential biomarker for predicting response to concurrent cetuximab and nivolumab in head and neck squamous cell carcinoma.Journal for immunotherapy of cancer · 2022Trial
- Decoding the mosaic of inflammatory bowel disease: Illuminating insights with single-cell RNA technology.Computational and structural biotechnology journal · 2024Review
- Multistep tumor genetic evolution and changes in immunogenicity trigger immune-mediated disease eradication in stage IV melanoma: lessons from a single case.Journal for immunotherapy of cancer · 2024Article
- Single-cell profiling reveals unique features of diabetogenic T cells in anti-PD-1-induced type 1 diabetes mice.The Journal of experimental medicine · 2023Article
- Identification and characterization of the T cell receptor (TCR) repertoire of the cynomolgus macaque (Macaca Fascicularis).BMC genomics · 2022Article
- TCR repertoire and transcriptional signatures of circulating tumour-associated T cells facilitate effective non-invasive cancer detection.Clinical and translational medicine · 2022Article
- Analysis of T cell receptor clonotypes in tumor microenvironment identifies shared cancer-type-specific signatures.Cancer immunology, immunotherapy : CII · 2022Article
- TCR-sequencing in cancer and autoimmunity: barcodes and beyond.Trends in immunology · 2022Review
- The Multifaceted Effects of Breast Cancer on Tumor-Draining Lymph Nodes.The American journal of pathology · 2021Review
- High-throughput and single-cell T cell receptor sequencing technologies.Nature methods · 2021Review
- Structures suggest an approach for converting weak self-peptide tumor antigens into superagonists for CD8 T cells in cancer.Proceedings of the National Academy of Sciences of the United States of America · 2021Article
- Analysis of T-Cell Receptor Repertoire in Transplantation: Fingerprint of T Cell-mediated Alloresponse.Frontiers in immunology · 2021Review
- T cell receptor sequencing in autoimmunity.Journal of life sciences (Westlake Village, Calif.) · 2020Article
- T cell receptor repertoires of ex vivo-expanded tumor-infiltrating lymphocytes from breast cancer patients.Immunologic research · 2020Observational
- T-cell receptor affinity in the age of cancer immunotherapy.Molecular carcinogenesis · 2020Review
- A facile technology for the high-throughput sequencing of the paired VH:VL and TCRβ:TCRα repertoires.Science advances · 2020Article
- Biophysicochemical motifs in T cell receptor sequences as a potential biomarker for high-grade serous ovarian carcinoma.PloS one · 2020Article
- Resident memory CD8+ T cells within cancer islands mediate survival in breast cancer patients.JCI insight · 2019Article
- The Genomic and Immune Landscapes of Lethal Metastatic Breast Cancer.Cell reports · 2019Article
- Biophysicochemical Motifs in T-cell Receptor Sequences Distinguish Repertoires from Tumor-Infiltrating Lymphocyte and Adjacent Healthy Tissue.Cancer research · 2019Article
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20 authors at 5 institutions in 1 country.
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Abstract
Infiltration of T cells in breast tumors correlates with improved survival of patients with breast cancer, despite relatively few mutations in these tumors. To determine if T-cell specificity can be harnessed to augment immunotherapies of breast cancer, we sought to identify the alpha-beta paired T-cell receptors (TCRs) of tumor-infiltrating lymphocytes shared between multiple patients. Because TCRs function as heterodimeric proteins, we used an emulsion-based RT-PCR assay to link and amplify TCR pairs. Using this assay on engineered T-cell hybridomas, we observed ∼85% accurate pairing fidelity, although TCR recovery frequency varied. When we applied this technique to patient samples, we found that for any given TCR pair, the dominant alpha- or beta-binding partner comprised ∼90% of the total binding partners. Analysis of TCR sequences from primary tumors showed about fourfold more overlap in tumor-involved relative to tumor-free sentinel lymph nodes. Additionally, comparison of sequences from both tumors of a patient with bilateral breast cancer showed 10% overlap. Finally, we identified a panel of unique TCRs shared between patients' tumors and peripheral blood that were not found in the peripheral blood of controls. These TCRs encoded a range of V, J, and complementarity determining region 3 (CDR3) sequences on the alpha-chain, and displayed restricted V-beta use. The nucleotides encoding these shared TCR CDR3s varied, suggesting immune selection of this response. Harnessing these T cells may provide practical strategies to improve the shared antigen-specific response to breast cancer.
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