ArticleGenome research2016
Gene discovery by chemical mutagenesis and whole-genome sequencing in Dictyostelium.
Article in Genome research, 2016. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 23 papers.
What it found
Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.
The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.
Who cites it
23 citing papers in PubMed, 31 citations in OpenAlex.
- Unlocking CRISPR-Cas9 editing for widely diverse Dictyostelid species.Molecular systems biology · 2026Article
- Genetic suppressor of autophagy defects in huntingtin null cells identified using a mutagenesis screen in Dictyostelium discoideum.BMC molecular and cell biology · 2026Article
- Mutual suppression between mutations in the Dictyostelium Greenbeard pathway restores wild-type development.BMC genomics · 2025Article
- A sequencing-based screening method identifies regulators of EGFR signaling from nonviable mutants inScience signaling · 2025Article
- Going against the family: Perturbation of a greenbeard pathway leads to falsebeard cheating.iScience · 2024Article
- The greenbeard gene tgrB1 regulates altruism and cheating in Dictyostelium discoideum.Nature communications · 2024Article
- Discovery of anti-infective compounds againstFrontiers in microbiology · 2024Article
- High-throughput approaches to understand and engineer bacteriophages.Trends in biochemical sciences · 2023Review
- CRISPR/Cas9-based genome-wide screening of Dictyostelium.Scientific reports · 2022Article
- The genetic architecture underlying prey-dependent performance in a microbial predator.Nature communications · 2022Article
- Comparative Genomics Reveals a Single Nucleotide Deletion inFrontiers in fungal biology · 2022Article
- Article
- Review
- Development of a Positive Selection High Throughput Genetic Screen inFrontiers in cell and developmental biology · 2021Article
- Coupling chemical mutagenesis to next generation sequencing for the identification of drug resistance mutations in Leishmania.Nature communications · 2019Article
- Cellular allorecognition and its roles in Dictyostelium development and social evolution.The International journal of developmental biology · 2019Review
- Article
- Whole-Genome Sequence Accuracy Is Improved by Replication in a Population of Mutagenized Sorghum.G3 (Bethesda, Md.) · 2018Article
- The polymorphic proteins TgrB1 and TgrC1 function as a ligand-receptor pair inJournal of cell science · 2017Article
- dictyExpress: a web-based platform for sequence data management and analytics in Dictyostelium and beyond.BMC bioinformatics · 2017Article
Corrections and comments
- Commented on by
Authors and funding
5 authors at 2 institutions in 2 countries.
Funding
Abstract
Whole-genome sequencing is a useful approach for identification of chemical-induced lesions, but previous applications involved tedious genetic mapping to pinpoint the causative mutations. We propose that saturation mutagenesis under low mutagenic loads, followed by whole-genome sequencing, should allow direct implication of genes by identifying multiple independent alleles of each relevant gene. We tested the hypothesis by performing three genetic screens with chemical mutagenesis in the social soil amoeba Dictyostelium discoideum Through genome sequencing, we successfully identified mutant genes with multiple alleles in near-saturation screens, including resistance to intense illumination and strong suppressors of defects in an allorecognition pathway. We tested the causality of the mutations by comparison to published data and by direct complementation tests, finding both dominant and recessive causative mutations. Therefore, our strategy provides a cost- and time-efficient approach to gene discovery by integrating chemical mutagenesis and whole-genome sequencing. The method should be applicable to many microbial systems, and it is expected to revolutionize the field of functional genomics in Dictyostelium by greatly expanding the mutation spectrum relative to other common mutagenesis methods.
Indexed as
Identifiers
What OpenQuestion holds
Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.