Evidence map›Paper›PMID 27307258›Full record

ArticlePlant physiology2016

Two Cytochrome P450 Monooxygenases Catalyze Early Hydroxylation Steps in the Potato Steroid Glycoalkaloid Biosynthetic Pathway.

Naoyuki Umemoto, Masaru Nakayasu, Kiyoshi Ohyama, Mari Yotsu-Yamashita, Masaharu Mizutani, Hikaru Seki, Kazuki Saito, Toshiya Muranaka

Open access · bronzeAbstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in Plant physiology, 2016. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 47 papers.

0numbers the graph read from it
0cells of the map it votes in
47citing papers in PubMed
13.5field-weighted citation impact, top 1% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

47 citing papers in PubMed, 114 citations in OpenAlex.

  1. Article
  2. A rare glycoalkaloid biosynthetic pathway from wild potato unlocks genetic routes to Colorado potato beetle resistance.Proceedings of the National Academy of Sciences of the United States of America · 2026
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  7. Biofortification of potato nutrition.Journal of advanced research · 2025
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  12. Development of phytochemical genomics: From decoding metabolome to functional genomics and biotechnology of plant metabolism.Proceedings of the Japan Academy. Series B, Physical and biological sciences · 2025
    Review
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  14. Review
  15. Disruption ofPlant biotechnology (Tokyo, Japan) · 2024
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4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

8 authors at 2 institutions in 1 country.

Naoyuki UmemotoCentral Laboratories for Key Technologies, Kirin Co., Kanazawa-ku, Yokohama, Kanagawa 236-0004, Japan (N.U.); RIKEN Center for Sustainable Resource Science, Tsurumi-ku, Yokohama, Kanagawa 230-0045, Japan (N.U., K.O., K.S.); Graduate School of Agricultural Science, Kobe University, Nada-ku, Kobe, Hyogo 657-8501, Japan (M.N., M.M.); Department of Chemistry and Materials Science, Tokyo Institute of Technology, Meguro-ku, Tokyo 152-8551, Japan (K.O.); Graduate School of Agricultural Science, Tohoku University, Aoba-ku, Sendai, Miyagi 981-8555, Japan (M.Y.-Y.); Department of Biotechnology, Graduate School of Engineering, Osaka University, Suita, Osaka 565-0871, Japan (H.S., T.M.); and Graduate School of Pharmaceutical Sciences, Chiba University, Chuo-ku, Chiba 260-8675, Japan (K.S.) naoyuki.umemoto@riken.jp.ORCID 0000-0001-5507-0848
Masaru NakayasuCentral Laboratories for Key Technologies, Kirin Co., Kanazawa-ku, Yokohama, Kanagawa 236-0004, Japan (N.U.); RIKEN Center for Sustainable Resource Science, Tsurumi-ku, Yokohama, Kanagawa 230-0045, Japan (N.U., K.O., K.S.); Graduate School of Agricultural Science, Kobe University, Nada-ku, Kobe, Hyogo 657-8501, Japan (M.N., M.M.); Department of Chemistry and Materials Science, Tokyo Institute of Technology, Meguro-ku, Tokyo 152-8551, Japan (K.O.); Graduate School of Agricultural Science, Tohoku University, Aoba-ku, Sendai, Miyagi 981-8555, Japan (M.Y.-Y.); Department of Biotechnology, Graduate School of Engineering, Osaka University, Suita, Osaka 565-0871, Japan (H.S., T.M.); and Graduate School of Pharmaceutical Sciences, Chiba University, Chuo-ku, Chiba 260-8675, Japan (K.S.).
Kiyoshi OhyamaCentral Laboratories for Key Technologies, Kirin Co., Kanazawa-ku, Yokohama, Kanagawa 236-0004, Japan (N.U.); RIKEN Center for Sustainable Resource Science, Tsurumi-ku, Yokohama, Kanagawa 230-0045, Japan (N.U., K.O., K.S.); Graduate School of Agricultural Science, Kobe University, Nada-ku, Kobe, Hyogo 657-8501, Japan (M.N., M.M.); Department of Chemistry and Materials Science, Tokyo Institute of Technology, Meguro-ku, Tokyo 152-8551, Japan (K.O.); Graduate School of Agricultural Science, Tohoku University, Aoba-ku, Sendai, Miyagi 981-8555, Japan (M.Y.-Y.); Department of Biotechnology, Graduate School of Engineering, Osaka University, Suita, Osaka 565-0871, Japan (H.S., T.M.); and Graduate School of Pharmaceutical Sciences, Chiba University, Chuo-ku, Chiba 260-8675, Japan (K.S.).
Mari Yotsu-YamashitaCentral Laboratories for Key Technologies, Kirin Co., Kanazawa-ku, Yokohama, Kanagawa 236-0004, Japan (N.U.); RIKEN Center for Sustainable Resource Science, Tsurumi-ku, Yokohama, Kanagawa 230-0045, Japan (N.U., K.O., K.S.); Graduate School of Agricultural Science, Kobe University, Nada-ku, Kobe, Hyogo 657-8501, Japan (M.N., M.M.); Department of Chemistry and Materials Science, Tokyo Institute of Technology, Meguro-ku, Tokyo 152-8551, Japan (K.O.); Graduate School of Agricultural Science, Tohoku University, Aoba-ku, Sendai, Miyagi 981-8555, Japan (M.Y.-Y.); Department of Biotechnology, Graduate School of Engineering, Osaka University, Suita, Osaka 565-0871, Japan (H.S., T.M.); and Graduate School of Pharmaceutical Sciences, Chiba University, Chuo-ku, Chiba 260-8675, Japan (K.S.).ORCID 0000-0002-5009-0409
Masaharu MizutaniCentral Laboratories for Key Technologies, Kirin Co., Kanazawa-ku, Yokohama, Kanagawa 236-0004, Japan (N.U.); RIKEN Center for Sustainable Resource Science, Tsurumi-ku, Yokohama, Kanagawa 230-0045, Japan (N.U., K.O., K.S.); Graduate School of Agricultural Science, Kobe University, Nada-ku, Kobe, Hyogo 657-8501, Japan (M.N., M.M.); Department of Chemistry and Materials Science, Tokyo Institute of Technology, Meguro-ku, Tokyo 152-8551, Japan (K.O.); Graduate School of Agricultural Science, Tohoku University, Aoba-ku, Sendai, Miyagi 981-8555, Japan (M.Y.-Y.); Department of Biotechnology, Graduate School of Engineering, Osaka University, Suita, Osaka 565-0871, Japan (H.S., T.M.); and Graduate School of Pharmaceutical Sciences, Chiba University, Chuo-ku, Chiba 260-8675, Japan (K.S.).ORCID 0000-0002-4321-0644
Hikaru SekiCentral Laboratories for Key Technologies, Kirin Co., Kanazawa-ku, Yokohama, Kanagawa 236-0004, Japan (N.U.); RIKEN Center for Sustainable Resource Science, Tsurumi-ku, Yokohama, Kanagawa 230-0045, Japan (N.U., K.O., K.S.); Graduate School of Agricultural Science, Kobe University, Nada-ku, Kobe, Hyogo 657-8501, Japan (M.N., M.M.); Department of Chemistry and Materials Science, Tokyo Institute of Technology, Meguro-ku, Tokyo 152-8551, Japan (K.O.); Graduate School of Agricultural Science, Tohoku University, Aoba-ku, Sendai, Miyagi 981-8555, Japan (M.Y.-Y.); Department of Biotechnology, Graduate School of Engineering, Osaka University, Suita, Osaka 565-0871, Japan (H.S., T.M.); and Graduate School of Pharmaceutical Sciences, Chiba University, Chuo-ku, Chiba 260-8675, Japan (K.S.).ORCID 0000-0002-6426-0368
Kazuki SaitoCentral Laboratories for Key Technologies, Kirin Co., Kanazawa-ku, Yokohama, Kanagawa 236-0004, Japan (N.U.); RIKEN Center for Sustainable Resource Science, Tsurumi-ku, Yokohama, Kanagawa 230-0045, Japan (N.U., K.O., K.S.); Graduate School of Agricultural Science, Kobe University, Nada-ku, Kobe, Hyogo 657-8501, Japan (M.N., M.M.); Department of Chemistry and Materials Science, Tokyo Institute of Technology, Meguro-ku, Tokyo 152-8551, Japan (K.O.); Graduate School of Agricultural Science, Tohoku University, Aoba-ku, Sendai, Miyagi 981-8555, Japan (M.Y.-Y.); Department of Biotechnology, Graduate School of Engineering, Osaka University, Suita, Osaka 565-0871, Japan (H.S., T.M.); and Graduate School of Pharmaceutical Sciences, Chiba University, Chuo-ku, Chiba 260-8675, Japan (K.S.).
Toshiya MuranakaCentral Laboratories for Key Technologies, Kirin Co., Kanazawa-ku, Yokohama, Kanagawa 236-0004, Japan (N.U.); RIKEN Center for Sustainable Resource Science, Tsurumi-ku, Yokohama, Kanagawa 230-0045, Japan (N.U., K.O., K.S.); Graduate School of Agricultural Science, Kobe University, Nada-ku, Kobe, Hyogo 657-8501, Japan (M.N., M.M.); Department of Chemistry and Materials Science, Tokyo Institute of Technology, Meguro-ku, Tokyo 152-8551, Japan (K.O.); Graduate School of Agricultural Science, Tohoku University, Aoba-ku, Sendai, Miyagi 981-8555, Japan (M.Y.-Y.); Department of Biotechnology, Graduate School of Engineering, Osaka University, Suita, Osaka 565-0871, Japan (H.S., T.M.); and Graduate School of Pharmaceutical Sciences, Chiba University, Chuo-ku, Chiba 260-8675, Japan (K.S.).ORCID 0000-0003-1058-2473
Tokyo Institute of Technology · JPKirin (Japan) · JP

Funding

No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

α-Solanine and α-chaconine, steroidal glycoalkaloids (SGAs) found in potato (Solanum tuberosum), are among the best-known secondary metabolites in food crops. At low concentrations in potato tubers, SGAs are distasteful; however, at high concentrations, SGAs are harmful to humans and animals. Here, we show that POTATO GLYCOALKALOID BIOSYNTHESIS1 (PGA1) and PGA2, two genes that encode cytochrome P450 monooxygenases (CYP72A208 and CYP72A188), are involved in the SGA biosynthetic pathway, respectively. The knockdown plants of either PGA1 or PGA2 contained very little SGA, yet vegetative growth and tuber production were not affected. Analyzing metabolites that accumulated in the plants and produced by in vitro enzyme assays revealed that PGA1 and PGA2 catalyzed the 26- and 22-hydroxylation steps, respectively, in the SGA biosynthetic pathway. The PGA-knockdown plants had two unique phenotypic characteristics: The plants were sterile and tubers of these knockdown plants did not sprout during storage. Functional analyses of PGA1 and PGA2 have provided clues for controlling both potato glycoalkaloid biosynthesis and tuber sprouting, two traits that can significantly impact potato breeding and the industry.

Indexed as

Biosynthetic PathwaysBreedingCrops, AgriculturalCytochrome P-450 Enzyme SystemGene SilencingHydroxylationPhenotypePhytosterolsPlant ProteinsPlant TubersSolanineSolanum tuberosumalpha-chaconinealpha-solanineCytochrome P-450 Enzyme SystemPhytosterolsPlant ProteinsSolanine

Identifiers

PMID27307258
PMCPMC4972264
OpenAlexW2433104495

What OpenQuestion holds

Textmetadata
Read underepoch 390

Registered trials

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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.