ArticleThe Journal of nutrition2016
Membrane Disordering by Eicosapentaenoic Acid in B Lymphomas Is Reduced by Elongation to Docosapentaenoic Acid as Revealed with Solid-State Nuclear Magnetic Resonance Spectroscopy of Model Membranes.
Article in The Journal of nutrition, 2016. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 8 papers.
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Who cites it
8 citing papers in PubMed, 10 citations in OpenAlex.
- DPA shows comparable chemotherapy sensitizing effects as EPA upon cellular incorporation in tumor cells.Oncotarget · 2019Article
- Regulation of monoamine transporters and receptors by lipid microdomains: implications for depression.Neuropsychopharmacology : official publication of the American College of Neuropsychopharmacology · 2018Review
- Mechanisms by Which Dietary Fatty Acids Regulate Mitochondrial Structure-Function in Health and Disease.Advances in nutrition (Bethesda, Md.) · 2018Review
- All n-3 PUFA are not the same: MD simulations reveal differences in membrane organization for EPA, DHA and DPA.Biochimica et biophysica acta. Biomembranes · 2018Article
- Effects of the polyunsaturated fatty acids, EPA and DHA, on hematological malignancies: a systematic review.Oncotarget · 2018Review
- DHA Modifies the Size and Composition of Raftlike Domains: A Solid-StateBiophysical journal · 2018Article
- Influence of omega-3 fatty acids on bovine luteal cell plasma membrane dynamics.Biochimica et biophysica acta. Biomembranes · 2017Article
- Characterization of lipid rafts in human platelets using nuclear magnetic resonance: A pilot study.Biochemistry and biophysics reports · 2017Article
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Authors and funding
6 authors at 2 institutions in 1 country.
Funding
Abstract
backgroundPlasma membrane organization is a mechanistic target of n-3 (ω-3) polyunsaturated fatty acids. Previous studies show that eicosapentaenoic acid (EPA; 20:5n-3) and docosahexaenoic acid (DHA; 22:6n-3) differentially disrupt plasma membrane molecular order to enhance the frequency and function of B lymphocytes. However, it is not known whether EPA and DHA affect the plasma membrane organization of B lymphomas differently to influence their function.
objectiveWe tested whether EPA and DHA had different effects on membrane order in B lymphomas and liposomes and studied their effects on B-lymphoma growth.
methodsB lymphomas were treated with 25 μmol EPA, DHA, or serum albumin control/L for 24 h. Membrane order was measured with fluorescence polarization, and cellular fatty acids (FAs) were analyzed with GC. Growth was quantified with a viability assay. (2)H nuclear magnetic resonance (NMR) studies were conducted on deuterated phospholipid bilayers.
resultsTreating Raji, Ramos, and RPMI lymphomas for 24 h with 25 μmol EPA or DHA/L lowered plasma membrane order by 10-40% relative to the control. There were no differences between EPA and DHA on membrane order for the 3 cell lines. FA analyses revealed complex changes in response to EPA or DHA treatment and a large fraction of EPA was converted to docosapentaenoic acid (DPA; 22:5n-3). NMR studies, which were used to understand why EPA and DHA had similiar membrane effects, showed that phospholipids containing DPA, similar to DHA, were more ordered than those containing EPA. Finally, treating B lymphomas with 25 μmol EPA or DHA/L did not increase the frequency of B lymphomas compared with controls.
conclusionsThe results establish that 25 μmol EPA and DHA/L equally disrupt membrane order and do not promote B lymphoma growth. The data open a new area of investigation, which is how EPA's conversion to DPA substantially moderates its influence on membrane properties.
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