ArticleMicrobiome2016
Two sampling methods yield distinct microbial signatures in the nasopharynges of asthmatic children.
Article in Microbiome, 2016. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 29 papers, 1 of them a synthesis that pooled it.
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Who cites it
29 citing papers in PubMed, 1 synthesis or guideline pooled it.
- Exploring the microbial landscape of the nasopharynx in children: a systematic review of studies using next generation sequencing.Frontiers in microbiomes · 2023Pooled it
- The respiratory tract virome: unravelling the role of viral dark matter in respiratory health and disease.European respiratory review : an official journal of the European Respiratory Society · 2025Review
- Dihydroartemisinin Modulates Enteric Glial Cell Heterogeneity to Alleviate Colitis.Advanced science (Weinheim, Baden-Wurttemberg, Germany) · 2024Article
- The nasal mycobiome of individuals with allergic rhinitis and asthma differs from that of healthy controls in composition, structure and function.Frontiers in microbiology · 2024Article
- Characterization of the oral mycobiome of Portuguese with allergic rhinitis and asthma.Current research in microbial sciences · 2024Article
- Bacterial Microbiota of Asthmatic Children and Preschool Wheezers' Airways-What Do We Know?Microorganisms · 2023Review
- A Review on the Nasal Microbiome and Various Disease Conditions for Newer Approaches to Treatments.Indian journal of otolaryngology and head and neck surgery : official publication of the Association of Otolaryngologists of India · 2023Article
- Realising respiratory microbiomic meta-analyses: time for a standardised framework.Microbiome · 2023Article
- Nasal Bacteriomes of Patients with Asthma and Allergic Rhinitis Show Unique Composition, Structure, Function and Interactions.Microorganisms · 2023Article
- Association Between Adult Antibiotic Use, Microbial Dysbiosis and Atopic Conditions - A Systematic Review.Journal of asthma and allergy · 2023Review
- Review
- The Environmental Microbiome, Allergic Disease, and Asthma.The journal of allergy and clinical immunology. In practice · 2022Article
- Article
- Integrated metabolic and microbial analysis reveals host-microbial interactions in IgE-mediated childhood asthma.Scientific reports · 2021Article
- Nasopharyngeal Swabs vs. Nasal Aspirates for Respiratory Virus Detection: A Systematic Review.Pathogens (Basel, Switzerland) · 2021Review
- Effects of nasopharyngeal microbiota in respiratory infections and allergies.Clinical and experimental pediatrics · 2021Article
- The Upper Airway Microbiota, Environmental Exposures, Inflammation, and Disease.Medicina (Kaunas, Lithuania) · 2021Review
- The microbiome of the nasopharynx.Journal of medical microbiology · 2021Review
- Bacterial Signatures of Paediatric Respiratory Disease: An Individual Participant Data Meta-Analysis.Frontiers in microbiology · 2021Article
- Nasal microbiome research in ANCA-associated vasculitis: Strengths, limitations, and future directions.Computational and structural biotechnology journal · 2021Review
Corrections and comments
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Authors and funding
3 authors.
Funding
Abstract
backgroundThe nasopharynx is a reservoir for pathogens associated with respiratory illnesses, such as asthma. Next-generation sequencing (NGS) has been used to characterize the nasopharyngeal microbiome during health and disease. Most studies so far have surveyed the nasopharynx as a whole; however, less is known about spatial variation (biogeography) in nasal microenvironments and how sampling techniques may capture that microbial diversity.
findingsWe used targeted 16S rRNA MiSeq sequencing and two different sampling strategies [nasal washes (NW) and nasal brushes (NB)] to characterize the nasopharyngeal microbiota in 30 asthmatic children. Nasal brushing is more abrasive than nasal washing and targeted the inner portion of the inferior turbinate. This region is expected to be different from other nasal microenvironments. Nasal washing is not spatially specific. Our 30 × 2 nasal microbiomes generated 1,474,497 sequences, from which we identified an average of 157 and 186 OTUs per sample in the NW and NB groups, respectively. Microbiotas from NB showed significantly higher alpha-diversity than microbiotas from NW. Similarly, both nasal microbiotas were distinct from each other (PCoA) and significantly differed in their community composition and abundance in at least 9 genera (effective size ≥1 %).
conclusionsNasopharyngeal microenvironments in asthmatic children contain microbiotas with different diversity and structure. Nasal washes and brushes capture that diversity differently. Future microbial studies of the nasopharynx need to be aware of potential spatial variation (biogeography).
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