ArticleScientific reports2016
Enhance nisin yield via improving acid-tolerant capability of Lactococcus lactis F44.
Article in Scientific reports, 2016. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 24 papers, 1 of them a synthesis that pooled it.
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Who cites it
24 citing papers in PubMed, 1 synthesis or guideline pooled it.
- Relationship between IL-10 gene -819C/T polymorphism and the risk of inflammatory bowel disease: a meta-analysis.African health sciences · 2016Pooled it
- The MarR family regulator RmaH underlies the trade-off between acid sensitivity and antibiotic tolerance inJournal of bacteriology · 2026Article
- Bactericidal and antibiofilm activity of lactic acid bacteria-derived cell free extracts against dairy-associated spoilage and pathogenic bacteria.Frontiers in microbiology · 2026Article
- New advances in biological preservation technology for aquatic products.NPJ science of food · 2025Review
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- Acid-tolerant bacteria and prospects in industrial and environmental applications.Applied microbiology and biotechnology · 2023Review
- Bioengineering of a Lactococcus lactis subsp. lactis strain enhances nisin production and bioactivity.PloS one · 2023Article
- Systems-Level Analysis of the Global Regulatory Mechanism of CodY in Lactococcus lactis Metabolism and Nisin Immunity Modulation.Applied and environmental microbiology · 2022Article
- CRISPR/Cas tools for enhancing the biopreservation ability of lactic acid bacteria in aquatic products.Frontiers in bioengineering and biotechnology · 2022Review
- Application of Biotechnology in Specific Spoilage Organisms of Aquatic Products.Frontiers in bioengineering and biotechnology · 2022Review
- Microbial production of small peptide: pathway engineering and synthetic biology.Microbial biotechnology · 2021Review
- Engineering Lactococcus lactis as a multi-stress tolerant biosynthetic chassis by deleting the prophage-related fragment.Microbial cell factories · 2020Article
- Higher nisin yield is reached with glutathione and pyruvate compared with heme in Lactococcus lactis N8.Brazilian journal of microbiology : [publication of the Brazilian Society for Microbiology] · 2020Article
- MiniACS synthetic biology · 2020Article
- d-Methionine and d-Phenylalanine Improve Lactococcus lactis F44 Acid Resistance and Nisin Yield by Governing Cell Wall Remodeling.Applied and environmental microbiology · 2020Article
- Safety Aspects of Genetically Modified Lactic Acid Bacteria.Microorganisms · 2020Review
- Co-production of Nisin and γ-Aminobutyric Acid by EngineeredFrontiers in microbiology · 2020Article
- Microbial response to acid stress: mechanisms and applications.Applied microbiology and biotechnology · 2020Review
- Systemic understanding of Lactococcus lactis response to acid stress using transcriptomics approaches.Journal of industrial microbiology & biotechnology · 2019Article
- Enhanced acid-stress tolerance in Lactococcus lactis NZ9000 by overexpression of ABC transporters.Microbial cell factories · 2019Article
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Authors and funding
7 authors.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Traditionally, nisin was produced industrially by using Lactococcus lactis in the neutral fermentation process. However, nisin showed higher activity in the acidic environment. How to balance the pH value for bacterial normal growth and nisin activity might be the key problem. In this study, 17 acid-tolerant genes and 6 lactic acid synthetic genes were introduced in L. lactis F44, respectively. Comparing to the 2810 IU/mL nisin yield of the original strain F44, the nisin titer of the engineered strains over-expressing hdeAB, ldh and murG, increased to 3850, 3979 and 4377 IU/mL, respectively. These engineered strains showed more stable intracellular pH value during the fermentation process. Improvement of lactate production could partly provide the extra energy for the expression of acid tolerance genes during growth. Co-overexpression of hdeAB, murG, and ldh(Z) in strain F44 resulted in the nisin titer of 4913 IU/mL. The engineered strain (ABGL) could grow on plates with pH 4.2, comparing to the surviving pH 4.6 of strain F44. The fed-batch fermentation showed nisin titer of the co-expression L. lactis strain could reach 5563 IU/mL with lower pH condition and longer cultivation time. This work provides a novel strategy of constructing robust strains for use in industry process.
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