ArticleTAG. Theoretical and applied genetics. Theoretische und angewandte Genetik2016
Development and validation of KASP assays for genes underpinning key economic traits in bread wheat.
Article in TAG. Theoretical and applied genetics. Theoretische und angewandte Genetik, 2016. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 203 papers.
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203 citing papers in PubMed.
- Genetic architecture and evolution of stripe rust resistance uncovered using diverse panels of wheat lines and North American Puccinia striiformis f. sp. tritici isolates.TAG. Theoretical and applied genetics. Theoretische und angewandte Genetik · 2026Article
- Post-Translational Control of TaFT1 by WAPO1 Ubiquitination Shapes Spike Architecture and Yield in Wheat.Plant biotechnology journal · 2026Article
- QTL Mapping for Leaf Rust Resistance in a Wheat Recombinant Inbred Line Population of Liang66-S/Hengmai28.Plants (Basel, Switzerland) · 2026Article
- Association Mapping of Seedling Resistance toPlants (Basel, Switzerland) · 2026Article
- Stable QTL Identification and KASP Marker Development for Grain Protein Content in Wheat Using a Linmai2/Zhong892 RIL Population.Plants (Basel, Switzerland) · 2026Article
- Genome-wide association mapping and haplotype analysis reveal the genetic architecture of sodicity tolerance in bread wheat (Triticum aestivum L.).Scientific reports · 2026Article
- Genetic dissection of gluten characteristics based on single- and multi-locus genome-wide association studies in wheat (Food chemistry. Molecular sciences · 2026Article
- Detection and Utilisation of KASPar SNP Marker Assays for Early Blight Resistance in Tomato.Current microbiology · 2026Article
- Molecular Labelling Tool for Cereal Genetic Resources Management Derived from Barley and Tetraploid Wheat Genebank-Genomics Projects.Plants (Basel, Switzerland) · 2026Article
- Article
- Genomic divergence, introgression and KASP fingerprinting panel development in five cultivar groups of sweet osmanthus.BMC plant biology · 2026Article
- Dissecting multi-rust resistance in wheat through genome-wide association study, haplotype analysis, and marker validation.The plant genome · 2026Article
- Current status and future perspective of genomics-assisted breeding in wheat (Breeding science · 2026Article
- Allelic variation of carotenoid biosynthesis and degradation genes across worldwide commercial durum wheat (Molecular breeding : new strategies in plant improvement · 2026Article
- Development of KASP Markers for Carnation Germplasm (or Cultivar) Fingerprinting Based on Reduced-Representation Genome Sequencing Technology.Plants (Basel, Switzerland) · 2026Article
- Uncovering the Genetic Basis of Grain Protein Content and Wet Gluten Content in Common Wheat (Plants (Basel, Switzerland) · 2026Article
- Uncover the genetic basis of processing quality related traits in common wheat (Frontiers in plant science · 2026Article
- Development and application of core collection, KASP markers and SNP-based DNA fingerprinting inFrontiers in plant science · 2026Article
- Development of KASP markers, SNP fingerprinting and population structure analysis ofFrontiers in plant science · 2026Article
- QTL detection, validation, candidate genes and KASP markers for pre-harvest sprouting tolerance in white grained common wheat.BMC plant biology · 2025Article
143 more citing papers are in PubMed but not listed here.
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Authors and funding
11 authors.
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Abstract
key messageWe developed and validated a robust marker toolkit for high-throughput and cost-effective screening of a large number of functional genes in wheat. Functional markers (FMs) are the most valuable markers for crop breeding programs, and high-throughput genotyping for FMs could provide an excellent opportunity to effectively practice marker-assisted selection while breeding cultivars. Here we developed and validated kompetitive allele-specific PCR (KASP) assays for genes that underpin economically important traits in bread wheat including adaptability, grain yield, quality, and biotic and abiotic stress resistances. In total, 70 KASP assays either developed in this study or obtained from public databases were validated for reliability in application. The validation of KASP assays were conducted by (a) comparing the assays with available gel-based PCR markers on 23 diverse wheat accessions, (b) validation of the derived allelic information using phenotypes of a panel comprised of 300 diverse cultivars from China and 13 other countries, and (c) additional testing, where possible, of the assays in four segregating populations. All KASP assays being reported were significantly associated with the relevant phenotypes in the cultivars panel and bi-parental populations, thus revealing potential application in wheat breeding programs. The results revealed 45 times superiority of the KASP assays in speed than gel-based PCR markers. KASP has recently emerged as single-plex high-throughput genotyping technology; this is the first report on high-throughput screening of a large number of functional genes in a major crop. Such assays could greatly accelerate the characterization of crossing parents and advanced lines for marker-assisted selection and can complement the inflexible, high-density SNP arrays. Our results offer a robust and reliable molecular marker toolkit that can contribute towards maximizing genetic gains in wheat breeding programs.
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