ArticleScientific reports2016
Identification of structurally closely related monosaccharide and disaccharide isomers by PMP labeling in conjunction with IM-MS/MS.
Article in Scientific reports, 2016. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 14 papers.
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14 citing papers in PubMed.
- Rice straw sample preparation protocol for cell wall major constituent analysis via Raman spectroscopy.STAR protocols · 2026Article
- Liquid chromatography-mass spectrometry approach for characterizing sucrose isomers in complex mono-floral honey.Analytical and bioanalytical chemistry · 2025Article
- Triple labeling of metabolites for metabolome analysis (TLEMMA): a stable isotope labeling approach for metabolite identification and network reconstruction.The Plant journal : for cell and molecular biology · 2025Article
- Analytical Strategies for Natural Mono- and Disaccharide Isomer Differentiation: Techniques and Applications.Drug design, development and therapy · 2025Review
- Mass Spectrometry Approach for Differentiation of Positional Isomers of Saccharides: Toward Direct Analysis of Rare Sugars.Analytical chemistry · 2023Article
- Distinctive carbohydrate profiles of black ginseng revealed by IM-MS combined with PMP labeling and multivariate data analysis.Current research in food science · 2022Article
- Combined whole cell wall analysis and streamlined in silico carbohydrate-active enzyme discovery to improve biocatalytic conversion of agricultural crop residues.Biotechnology for biofuels · 2021Review
- Analysis of oligosaccharides fromJournal of ginseng research · 2020Article
- Differentiation and identification of ginsenoside structural isomers by two-dimensional mass spectrometry combined with statistical analysis.Journal of ginseng research · 2019Article
- Recent advances in ion mobility-mass spectrometry for improved structural characterization of glycans and glycoconjugates.Current opinion in chemical biology · 2018Review
- Characterization of Conformational Ensembles of Protonated N-glycans in the Gas-Phase.Scientific reports · 2018Article
- Fast and Effective Ion Mobility-Mass Spectrometry Separation of d-Amino-Acid-Containing Peptides.Analytical chemistry · 2017Article
- Characterization of chiral amino acids from different milk origins using ultra-performance liquid chromatography coupled to ion-mobility mass spectrometry.Scientific reports · 2017Article
- Qualitative and Quantitative Characterization of Monosaccharide Components ofJournal of analytical methods in chemistry · 2017Article
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8 authors.
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Abstract
It remains particularly difficult for gaining unambiguous information on anomer, linkage, and position isomers of oligosaccharides using conventional mass spectrometry (MS) methods. In our laboratory, an ion mobility (IM) shift strategy was employed to improve confidence in the identification of structurally closely related disaccharide and monosaccharide isomers using IMMS. Higher separation between structural isomers was achieved using 1-phenyl-3-methyl-5-pyrazolone (PMP) derivatization in comparison with phenylhydrazine (PHN) derivatization. Furthermore, the combination of pre-IM fragmentation of PMP derivatives provided sufficient resolution to separate the isomers not resolved in the IMMS. To chart the structural variation observed in IMMS, the collision cross sections (CCSs) for the corresponding ions were measured. We analyzed nine disaccharide and three monosaccharide isomers that differ in composition, linkages, or configuration. Our data show that coexisting carbohydrate isomers can be identified by the PMP labeling technique in conjunction with ion-mobility separation and tandem mass spectrometry. The practical application of this rapid and effective method that requires only small amounts of sample is demonstrated by the successful analysis of water-soluble ginseng extract. This demonstrated the potential of this method to measure a variety of heterogeneous sample mixtures, which may have an important impact on the field of glycomics.
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