ArticleAnalytical chemistry2016
Instrument-Free Point-of-Care Molecular Detection of Zika Virus.
Article in Analytical chemistry, 2016. The graph could read no effect estimate from its abstract, so it casts no vote on the map. An erratum has been issued. Cited by 129 papers.
What it found
Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.
The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.
Who cites it
129 citing papers in PubMed, 315 citations in OpenAlex.
- Naked-Eye Visual Loop-Mediated Isothermal Amplification Method for the Rapid Detection of Chikungunya Virus.Methods in molecular biology (Clifton, N.J.) · 2027Article
- Amplification of RNA for identification of Zika and HCV in whole blood.Science advances · 2026Article
- Extraoral Detection of Biomarkers and Pathogens in Saliva: Comprehensive, Panoramic Review.Biosensors · 2026Review
- Trends of nucleic acid - based point-of-care diagnostics for infectious diseases.Journal of biological engineering · 2026Review
- Thermally Responsive Alkane Partitions and a Magnetofluidic Assay for Point-of-Sample Detection of Viruses in Wastewater.Biosensors · 2025Article
- A portable low-cost polymerase chain reaction device.HardwareX · 2025Article
- Point-of-Care Diagnostics Using Self-heating Elements from Smart Food Packaging: Moving Towards Instrument-Free Nucleic Acid-Based Detection.Molecular diagnosis & therapy · 2025Review
- Chemical Heating for Minimally Instrumented Point-of-Care (POC) Molecular Diagnostics.Biosensors · 2024Review
- Ultrasensitive Surface-Enhanced Raman Scattering Platform for Protein Detection via Active Delivery to Nanogaps as a Hotspot.ACS nano · 2024Article
- Review
- An Ultra-Compact and Low-Cost LAMP-Based Virus Detection Device.Sensors (Basel, Switzerland) · 2024Article
- Article
- A paradigm shift in the detection of bloodborne pathogens: conventional approaches to recent detection techniques.EXCLI journal · 2024Review
- Maximising Affordability of Real-Time Colorimetric LAMP Assays.Micromachines · 2023Article
- Loop mediated isothermal amplification as a molecular diagnostic assay: Application and evaluation for detection of EnterohaemorrhagicPractical laboratory medicine · 2023Article
- Mayaro virus detection by integrating sample preparation with isothermal amplification in portable devices.Analytical and bioanalytical chemistry · 2023Article
- Rapid On-Site Detection of SARS-CoV-2 Using RT-LAMP Assay with a Portable Low-Cost Device.Biosensors · 2023Article
- Trends of respiratory virus detection in point-of-care testing: A review.Analytica chimica acta · 2023Review
- Advances in point-of-care genetic testing for personalized medicine applications.Biomicrofluidics · 2023Review
- Latest Advances in Arbovirus Diagnostics.Microorganisms · 2023Review
69 more citing papers are in PubMed but not listed here.
Corrections and comments
- Erratum issued
Authors and funding
6 authors at 1 institution in 1 country.
Funding
Abstract
The recent outbreak of Zika virus (ZIKV) infection in the Americas and its devastating impact on fetal development have prompted the World Health Organization (WHO) to declare the ZIKV pandemic as a Public Health Emergency of International Concern. Rapid and reliable diagnostics for ZIKV are vital because ZIKV-infected individuals display no symptoms or nonspecific symptoms similar to other viral infections. Because immunoassays lack adequate sensitivity and selectivity and are unable to identify active state of infection, molecular diagnostics are an effective means to detect ZIKV soon after infection and throughout pregnancy. We report on a highly sensitive reverse-transcription loop-mediated, isothermal amplification (RT-LAMP) assay for rapid detection of ZIKV and its implementation in a simple, easy-to-use, inexpensive, point-of-care (POC) disposable cassette that carries out all the unit operations from sample introduction to detection. For thermal control of the cassette, we use a chemically heated cup without a need for electrical power. Amplification products are detected with leuco crystal violet (LCV) dye by eye without a need for instrumentation. We demonstrated the utility of our POC diagnostic system by detecting ZIKV in oral samples with sensitivity of 5 plaque-forming units (PFU) in less than 40 min. Our system is particularly suitable for resource-poor settings, where centralized laboratory facilities, funds, and trained personnel are in short supply, and for use in doctors' offices, clinics, and at home.
Indexed as
Identifiers
What OpenQuestion holds
Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.