ArticleEnvironmental science and pollution research international2016
Quantitative detection of viable helminth ova from raw wastewater, human feces, and environmental soil samples using novel PMA-qPCR methods.
Article in Environmental science and pollution research international, 2016. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 14 papers.
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14 citing papers in PubMed, 30 citations in OpenAlex.
- WASH for WORMS: A Cluster-Randomized Controlled Trial of the Impact of a Community Integrated Water, Sanitation, and Hygiene and Deworming Intervention on Soil-Transmitted Helminth Infections.The American journal of tropical medicine and hygiene · 2019Trial
- Assessment of Environmental Contamination With Soil-Transmitted Helminth Eggs From Human and Animal Faeces in Southern Côte d'Ivoire: A Rapid Approach for Identifying High-Risk Communities.Journal of parasitology research · 2026Article
- Molecular surveillance of intestinal parasites and Acanthamoeba species in soils from outdoor built environments in rural northwestern Argentina.Parasite (Paris, France) · 2026Article
- Parasites in Sewage: Legal Requirements and Diagnostic Tools.Pathogens (Basel, Switzerland) · 2025Review
- Molecular Testing of Environmental Samples as a Potential Source to Estimate Parasite Infection.Tropical medicine and infectious disease · 2024Article
- Article
- Potentials and challenges in the isolation and detection of ascarid eggs in complex environmental matrices.Food and waterborne parasitology · 2022Article
- Assessment of environmental contamination with soil-transmitted helminths life stages at school compounds, households and open markets in Jimma Town, Ethiopia.PLoS neglected tropical diseases · 2022Article
- An Optimized Method to Assess ViableFrontiers in microbiology · 2020Article
- The role of water, sanitation and hygiene interventions in reducing soil-transmitted helminths: interpreting the evidence and identifying next steps.Parasites & vectors · 2019Review
- New insights into the kinetics of bacterial growth and decay in pig manure-wheat straw aerobic composting based on an optimized PMA-qPCR method.Microbial biotechnology · 2019Article
- Detecting and enumerating soil-transmitted helminth eggs in soil: New method development and results from field testing in Kenya and Bangladesh.PLoS neglected tropical diseases · 2017Article
- Quantitative PCR and Digital PCR for Detection ofBioMed research international · 2017Article
- Transmission of waterborne parasites in the Association of Southeast Asian Nations (ASEAN): Overview and direction forward.Food and waterborne parasitologyReview
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Authors and funding
10 authors at 5 institutions in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
In this study, we have evaluated the efficacy of propidium monoazide quantitative polymerase chain reaction (PMA-qPCR) to differentiate between viable and non-viable Ancylostoma caninum ova. The newly developed method was validated using raw wastewater seeded with known numbers of A. caninum ova. Results of this study confirmed that PMA-qPCR has resulted in average of 88 % reduction (P < 0.05) in gene copy numbers for 50 % viable +50 % non-viable when compared with 100 % viable ova. A reduction of 100 % in gene copies was observed for 100 % non-viable ova when compared with 100 % viable ova. Similar reductions (79-80 %) in gene copies were observed for A. caninum ova-seeded raw wastewater samples (n = 18) collected from wastewater treatment plants (WWTPs) A and B. The newly developed PMA-qPCR method was applied to determine the viable ova of different helminths (A. caninum, A. duodenale, Necator americanus and Ascaris lumbricoides) in raw wastewater, human fecal and soil samples. None of the unseeded wastewater samples were positive for the above-mentioned helminths. N. americanus and A. lumbricoides ova were found in unseeded human fecal and soil samples. For the unseeded human fecal samples (1 g), an average gene copy concentration obtained from qPCR and PMA-qPCR was found to be similar (6.8 × 10(5) ± 6.4 × 10(5) and 6.3 × 10(5) ± 4.7 × 10(5)) indicating the presence of viable N. americanus ova. Among the 24 unseeded soil samples tested, only one was positive for A. lumbricoides. The mean gene copy concentration in the positively identified soil sample was 1.0 × 10(5) ± 1.5 × 10(4) (determined by qPCR) compared to 4.9 × 10(4) ± 3.7 × 10(3) (determined by PMA-qPCR). The newly developed PMA-qPCR methods were able to detect viable helminth ova from wastewater and soil samples and could be adapted for health risk assessment.
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