Evidence map›Paper›PMID 27305977›Full record

ArticleEpidemiology and infection2016

Genetic relatedness of selected clinical Vibrio cholerae O139 isolates from the southern coastal area of China over a 20-year period.

B S Li, Y Xiao, D C Wang, H L Tan, B X Ke, D M He, C W Ke, Y H Zhang

Abstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in Epidemiology and infection, 2016. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 4 papers.

0numbers the graph read from it
0cells of the map it votes in
4citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

4 citing papers in PubMed.

  1. Review
  2. Article
  3. New Variant of Vibrio cholerae O139 in Odisha, India.Journal of clinical microbiology · 2019
    Article
  4. Review
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

8 authors.

B S LiKey Laboratory for Repository and Application of Pathogenic Microbiology,Research Center for Pathogens Detection Technology of Emerging Infectious Diseases,Guangdong Provincial Center for Disease Control and Prevention,Guangzhou,China.
Y XiaoSchool of Public Health and Tropical Medicine,Southern Medical University,Guangzhou,China.
D C WangState Key Laboratory of Infectious Disease Prevention and Control,National Institute for Communicable Disease Control and Prevention,ChinaCDC,Beijing,China.
H L TanKey Laboratory for Repository and Application of Pathogenic Microbiology,Research Center for Pathogens Detection Technology of Emerging Infectious Diseases,Guangdong Provincial Center for Disease Control and Prevention,Guangzhou,China.
B X KeKey Laboratory for Repository and Application of Pathogenic Microbiology,Research Center for Pathogens Detection Technology of Emerging Infectious Diseases,Guangdong Provincial Center for Disease Control and Prevention,Guangzhou,China.
D M HeKey Laboratory for Repository and Application of Pathogenic Microbiology,Research Center for Pathogens Detection Technology of Emerging Infectious Diseases,Guangdong Provincial Center for Disease Control and Prevention,Guangzhou,China.
C W KeKey Laboratory for Repository and Application of Pathogenic Microbiology,Research Center for Pathogens Detection Technology of Emerging Infectious Diseases,Guangdong Provincial Center for Disease Control and Prevention,Guangzhou,China.
Y H ZhangKey Laboratory for Repository and Application of Pathogenic Microbiology,Research Center for Pathogens Detection Technology of Emerging Infectious Diseases,Guangdong Provincial Center for Disease Control and Prevention,Guangzhou,China.

Funding

No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

Vibrio cholerae O139 emerged as a causative agent of epidemic cholera in 1992 in India and Bangladesh, and was subsequently reported in China in 1993. The genetic relatedness and molecular characteristics of V. cholerae O139 in Guangdong Province, located in the southern coastal area of China, remains undetermined. In this study, we investigated 136 clinical V. cholerae O139 isolates from 1993 to 2013 in Guangdong. By conventional PCR, 123 (90·4%) isolates were positive for ctxB, ace and zot. Sequencing of the positive amplicons indicated 113 (91·7%) isolates possessed the El Tor allele of ctxB (genotype 3); seven carried the classical ctxB type (genotype 1) and three harboured a novel ctxB type (genotype 5). With respect to tcpA, 123 (90·4%) isolates were positive for the El Tor allele. In addition, pulsed-field gel electrophoresis (with NotI digestion) differentiated the isolates into clusters A and B. Cluster A contained seven of the non-toxigenic isolates from 1998 to 2000; another six non-toxigenic isolates (from 1998 and 2007) and all of the toxigenic isolates formed cluster B. Our results suggest that over a 20-year period, the predominant O139 clinical isolates have maintained a relatively tight clonal structure, although some genetic variance and shift has occurred. Our data highlight the persistence of toxigenic V. cholerae O139 in clinical settings in the southern coastal area of China.

Indexed as

Genetic VariationAmino Acid SequenceChinaCholeraCholera ToxinElectrophoresis, Gel, Pulsed-FieldGenotypePhylogenyPolymerase Chain ReactionSequence AlignmentVibrio cholerae O139Cholera ToxinBacterial typingclinical microbiologyepidemicsVibrio cholerae

Identifiers

PMID27305977
PMCPMC9150482

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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.