ArticleAngewandte Chemie (International ed. in English)2016
A Genetically Encoded β-Lactamase Reporter for Ultrasensitive (129) Xe NMR in Mammalian Cells.
Article in Angewandte Chemie (International ed. in English), 2016. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 37 papers.
What it found
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The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
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Who cites it
37 citing papers in PubMed, 55 citations in OpenAlex.
- Continuous Delivery of Hyperpolarized Xenon-129 Gas Using a "Stopped-Flow" Clinical-Scale Cryogen-Free Hyperpolarizer.Analytical chemistry · 2025Article
- UltrasensitiveAdvanced science (Weinheim, Baden-Wurttemberg, Germany) · 2025Review
- Article
- Hyperpolarized Xenon-129 Chemical Exchange Saturation Transfer (HyperCEST) Molecular Imaging: Achievements and Future Challenges.International journal of molecular sciences · 2024Review
- Biogenic Imaging Contrast Agents.Advanced science (Weinheim, Baden-Wurttemberg, Germany) · 2023Review
- Local Xenon-Protein Interaction Produces Global Conformational Change and Allosteric Inhibition in Lysozyme.Biochemistry · 2023Article
- Protein and peptide engineering for chemical exchange saturation transfer imaging in the age of synthetic biology.NMR in biomedicine · 2023Review
- Rational design of a genetically encoded NMR zinc sensor.Chemical science · 2023Article
- Programming xenon diffusion in maltose-binding protein.Biophysical journal · 2022Article
- Pilot Quality-Assurance Study of a Third-Generation Batch-Mode Clinical-Scale Automated Xenon-129 Hyperpolarizer.Molecules (Basel, Switzerland) · 2022Article
- Ultrasensitive molecular building block for biothiol NMR detection at picomolar concentrations.iScience · 2021Article
- Coloring ultrasensitive MRI with tunable metal-organic frameworks.Chemical science · 2021Article
- Mapping of Absolute Host Concentration and Exchange Kinetics of Xenon Hyper-CEST MRI Agents.Pharmaceuticals (Basel, Switzerland) · 2021Article
- Cryptophane-xenon complexes forRSC advances · 2021Article
- Binding site exchange kinetics revealed through efficient spin-spin dephasing of hyperpolarizedChemical science · 2020Article
- Molecular Sensing with Host Systems for HyperpolarizedMolecules (Basel, Switzerland) · 2020Review
- Article
- Paramagnetic Organocobalt Capsule Revealing Xenon Host-Guest Chemistry.Inorganic chemistry · 2020Article
- Detecting protein-protein interactions by Xe-129 NMR.Chemical communications (Cambridge, England) · 2020Article
- Hyperpolarized Xe NMR signal advancement by metal-organic framework entrapment in aqueous solution.Proceedings of the National Academy of Sciences of the United States of America · 2020Article
Corrections and comments
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Authors and funding
5 authors at 2 institutions in 1 country.
Funding
Abstract
Molecular imaging holds considerable promise for elucidating biological processes in normal physiology as well as disease states, but requires noninvasive methods for identifying analytes at sub-micromolar concentrations. Particularly useful are genetically encoded, single-protein reporters that harness the power of molecular biology to visualize specific molecular processes, but such reporters have been conspicuously lacking for in vivo magnetic resonance imaging (MRI). Herein, we report TEM-1 β-lactamase (bla) as a single-protein reporter for hyperpolarized (HP) (129) Xe NMR, with significant saturation contrast at 0.1 μm. Xenon chemical exchange saturation transfer (CEST) interactions with the primary allosteric site in bla give rise to a unique saturation peak at 255 ppm, well removed (≈60 ppm downfield) from the (129) Xe-H2 O peak. Useful saturation contrast was also observed for bla expressed in bacterial cells and mammalian cells.
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What OpenQuestion holds
Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.