ArticleInvestigative ophthalmology & visual science2016
The Phosphoinosotide 3-Kinase Catalytic Subunit p110α is Required for Normal Lens Growth.
Article in Investigative ophthalmology & visual science, 2016. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 18 papers.
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Who cites it
18 citing papers in PubMed, 18 citations in OpenAlex.
- Lens aging and disease: Molecular mechanisms, functional consequences, and pharmacological implications.Progress in retinal and eye research · 2026Review
- FoxO1 promotes high glucose-induced inflammation and cataract formation via JAK1/STAT1.Graefe's archive for clinical and experimental ophthalmology = Albrecht von Graefes Archiv fur klinische und experimentelle Ophthalmologie · 2025Article
- Combinatorial genetic manipulation of Cx50, PI3K and PTEN alters postnatal mouse lens growth and homeostasis.Frontiers in ophthalmology · 2025Article
- Altered Cell Clusters and Upregulated Aqp1 in Connexin 50 Knockout Lens Epithelium.Investigative ophthalmology & visual science · 2024Article
- Single-Cell RNA Sequencing Analysis of the Early Postnatal Mouse Lens Epithelium.Investigative ophthalmology & visual science · 2023Article
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- The Pro-Fibrotic Response to Lens Injury Is Signaled in a PI3K Isoform-Specific Manner.Biomolecules · 2022Article
- Suppression of PI3K signaling is linked to autophagy activation and the spatiotemporal induction of the lens organelle free zone.Experimental cell research · 2022Article
- Jack of all trades, master of each: the diversity of fibroblast growth factor signalling in eye development.Open biology · 2022Review
- Lens Connexin Channels Show Differential Permeability to Signaling Molecules.International journal of molecular sciences · 2020Review
- Connexin43 and connexin50 channels exhibit different permeability to the second messenger inositol triphosphate.Scientific reports · 2020Article
- Connexin 50-R205G Mutation Perturbs Lens Epithelial Cell Proliferation and Differentiation.Investigative ophthalmology & visual science · 2020Article
- The Ciliary Muscle and Zonules of Zinn Modulate Lens Intracellular Hydrostatic Pressure Through Transient Receptor Potential Vanilloid Channels.Investigative ophthalmology & visual science · 2019Article
- Lens Connexin Channels Have Differential Permeability to the Second Messenger cAMP.Investigative ophthalmology & visual science · 2019Article
- Lens differentiation is controlled by the balance between PDGF and FGF signaling.PLoS biology · 2019Article
- The lens growth process.Progress in retinal and eye research · 2017Review
- A full lifespan model of vertebrate lens growth.Royal Society open science · 2017Article
Corrections and comments
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Authors and funding
6 authors at 2 institutions in 2 countries.
Funding
Abstract
purposeSignal transduction pathways influence lens growth, but little is known about the role(s) of the class 1A phosphoinositide 3-kinases (PI3Ks). To further investigate how signaling regulates lens growth, we generated and characterized mice in which the p110α and p110β catalytic subunits of PI3K were conditionally deleted in the mouse lens.
methodsFloxed alleles of the catalytic subunits of PI3K were conditionally deleted in the lens by using MLR10-cre transgenic mice. Lenses of age-matched animals were dissected and photographed. Postnatal lenses were fixed, paraffin embedded, sectioned, and stained with hematoxylin-eosin. Cell proliferation was quantified by labeling S-phase cells in intact lenses with 5-ethynyl-2'-deoxyuridine. Protein kinase B (AKT) activation was examined by Western blotting.
resultsLens-specific deletion of p110α resulted in a significant reduction of eye and lens size, without compromising lens clarity. Conditional knockout of p110β had no effect on lens size or clarity, and deletion of both the p110α and p110β subunits resulted in a phenotype that resembled the p110α single-knockout phenotype. Levels of activated AKT were decreased more in p110α- than in p110β-deficient lenses. A significant reduction in proliferating cells in the germinative zone was observed on postnatal day 0 in p110α knockout mice, which was temporally correlated with decreased lens volume.
conclusionsThese data suggest that the class 1A PI3K signaling pathway plays an important role in the regulation of lens size by influencing the extent and spatial location of cell proliferation in the perinatal period.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.