ArticlePloS one2016
RPL13A and EEF1A1 Are Suitable Reference Genes for qPCR during Adipocyte Differentiation of Vascular Stromal Cells from Patients with Different BMI and HOMA-IR.
Article in PloS one, 2016. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 19 papers.
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19 citing papers in PubMed, 35 citations in OpenAlex.
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- Expression patterns of housekeeping genes and tissue-specific genes in black goats across multiple tissues.Scientific reports · 2024Article
- Advanced breast cancer diagnosis: Multiplex RT-qPCR for precise typing and angiogenesis profiling.Biochemistry and biophysics reports · 2024Article
- Engineered allele substitution at PPARGC1A rs8192678 alters human white adipocyte differentiation, lipogenesis, and PGC-1α content and turnover.Diabetologia · 2023Article
- A Strategy for the Selection of RT-qPCR Reference Genes Based on Publicly Available Transcriptomic Datasets.Biomedicines · 2023Article
- Validation of reference genes for the normalization of the RT-qPCR in peripheral blood mononuclear cells of septic patients.Heliyon · 2023Article
- Pulmonary health effects of wintertime particulate matter from California and China following repeated exposure and cessation.Toxicology letters · 2022Article
- HDAC inhibitors improve CRISPR-mediated HDR editing efficiency in iPSCs.Science China. Life sciences · 2021Article
- Screening of Reference Genes for RT-qPCR in Chicken Adipose Tissue and Adipocytes.Frontiers in physiology · 2021Article
- Identification of qPCR reference genes suitable for normalising gene expression in the developing mouse embryo.Wellcome open research · 2021Article
- Suppression of MKL1 promotes adipocytic differentiation and reduces the proliferation of myxoid liposarcoma cells.Oncology letters · 2020Article
- Sensitive and quantitative probing of pseudouridine modification in mRNA and long noncoding RNA.RNA (New York, N.Y.) · 2019Article
- Expression of the receptor of advanced glycation end-products (RAGE) and membranal location in peripheral blood mononuclear cells (PBMC) in obesity and insulin resistance.Iranian journal of basic medical sciences · 2019Article
- Reliable reference genes for expression analysis of proliferating and adipogenically differentiating human adipose stromal cells.Cellular & molecular biology letters · 2019Article
- Identification of appropriate reference genes for local immune-related studies in Morada Nova sheep infected with Haemonchus contortus.Molecular biology reports · 2018Article
- Article
- Evaluation of candidate reference genes for RT-qPCR studies in three metabolism related tissues of mice after caloric restriction.Scientific reports · 2016Article
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Authors and funding
11 authors at 7 institutions in 2 countries.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Real-time or quantitative PCR (qPCR) is a useful technique that requires reliable reference genes for data normalization in gene expression analysis. Adipogenesis is among the biological processes suitable for this technique. The selection of adequate reference genes is essential for qPCR gene expression analysis of human Vascular Stromal Cells (hVSCs) during their differentiation into adipocytes. To the best of our knowledge, there are no studies validating reference genes for the analyses of visceral and subcutaneous adipose tissue hVSCs from subjects with different Body Mass Index (BMI) and Homeostatic Model Assessment of Insulin Resistance (HOMA-IR) index. The present study was undertaken to analyze this question. We first analyzed the stability of expression of five potential reference genes: CYC, GAPDH, RPL13A, EEF1A1, and 18S ribosomal RNA, during in vitro adipogenic differentiation, in samples from these types of patients. The expression of RPL13A and EEF1A1 was not affected by differentiation, thus being these genes the most stable candidates, while CYC, GAPDH, and 18S were not suitable for this sort of analysis. This work highlights that RPL13A and EEF1A1 are good candidates as reference genes for qPCR analysis of hVSCs differentiation into adipocytes from subjects with different BMI and HOMA-IR.
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