Evidence map›Paper›PMID 27303623›Full record

ArticleF1000Research2015

Cannabis microbiome sequencing reveals several mycotoxic fungi native to dispensary grade Cannabis flowers.

Kevin McKernan, Jessica Spangler, Lei Zhang, Vasisht Tadigotla, Yvonne Helbert, Theodore Foss, Douglas Smith

Abstract read
In one paragraph

Article in F1000Research, 2015. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 28 papers, 2 of them syntheses that pooled it.

0numbers the graph read from it
0cells of the map it votes in
28citing papers in PubMed, 2 pooled it
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

28 citing papers in PubMed, 2 syntheses or guidelines pooled it.

  1. Pooled it
  2. Pooled it
  3. Toxins · 2026
    Article
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  5. Endophytes inPlants (Basel, Switzerland) · 2025
    Review
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  7. Challenges toInternational journal of molecular sciences · 2023
    Article
  8. Article
  9. Review
  10. Article
  11. Investigation of microorganisms in cannabis after heating in a commercial vaporizer.Frontiers in cellular and infection microbiology · 2022
    Article
  12. Article
  13. Review
  14. Article
  15. Article
  16. Article
  17. Article
  18. Article
  19. Article
  20. Review
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

7 authors.

Kevin McKernanMedicinal Genomics Corporation, Woburn, MA, USA.
Jessica SpanglerMedicinal Genomics Corporation, Woburn, MA, USA.
Lei ZhangMedicinal Genomics Corporation, Woburn, MA, USA.
Vasisht TadigotlaMedicinal Genomics Corporation, Woburn, MA, USA.
Yvonne HelbertMedicinal Genomics Corporation, Woburn, MA, USA.
Theodore FossMedicinal Genomics Corporation, Woburn, MA, USA.
Douglas SmithMedicinal Genomics Corporation, Woburn, MA, USA.

Funding

No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

The Center for Disease Control estimates 128,000 people in the U.S. are hospitalized annually due to food borne illnesses. This has created a demand for food safety testing targeting the detection of pathogenic mold and bacteria on agricultural products. This risk extends to medical Cannabis and is of particular concern with inhaled, vaporized and even concentrated Cannabis products . As a result, third party microbial testing has become a regulatory requirement in the medical and recreational Cannabis markets, yet knowledge of the Cannabis microbiome is limited. Here we describe the first next generation sequencing survey of the fungal communities found in dispensary based Cannabis flowers by ITS2 sequencing, and demonstrate the sensitive detection of several toxigenic Penicillium and Aspergillus species, including P. citrinum and P. paxilli, that were not detected by one or more culture-based methods currently in use for safety testing.

Indexed as

CannabidiolCannabisCitrininCultureMicrobiomeMycotoxinsNext generation sequencingPaxillineqPCR

Identifiers

PMID27303623
PMCPMC4897766

What OpenQuestion holds

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LicenceCC BY
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.