ArticleFrontiers in cellular neuroscience2016
Super Resolution Imaging of Genetically Labeled Synapses in Drosophila Brain Tissue.
Article in Frontiers in cellular neuroscience, 2016. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 9 papers.
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Who cites it
9 citing papers in PubMed, 17 citations in OpenAlex.
- Genetic regulation of central synapse formation and organization in Drosophila melanogaster.Genetics · 2022Review
- A pairwise distance distribution correction (DDC) algorithm to eliminate blinking-caused artifacts in SMLM.Nature methods · 2021Article
- Super-resolution imaging of flat-mounted whole mouse cornea.Experimental eye research · 2021Article
- Statistical analysis of 3D localisation microscopy images for quantification of membrane protein distributions in a platelet clot model.PLoS computational biology · 2020Article
- A Picture Worth a Thousand Molecules-Integrative Technologies for Mapping Subcellular Molecular Organization and Plasticity in Developing Circuits.Frontiers in synaptic neuroscience · 2020Article
- Rapid single-wavelength lightsheet localization microscopy for clarified tissue.Nature communications · 2019Article
- A method for estimating relative changes in the synaptic density in Drosophila central nervous system.BMC neuroscience · 2018Article
- Active Zone Scaffold Protein Ratios Tune Functional Diversity across Brain Synapses.Cell reports · 2018Article
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4 authors at 1 institution in 1 country.
Funding
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Abstract
Understanding synaptic connectivity and plasticity within brain circuits and their relationship to learning and behavior is a fundamental quest in neuroscience. Visualizing the fine details of synapses using optical microscopy remains however a major technical challenge. Super resolution microscopy opens the possibility to reveal molecular features of synapses beyond the diffraction limit. With direct stochastic optical reconstruction microscopy, dSTORM, we image synaptic proteins in the brain tissue of the fruit fly, Drosophila melanogaster. Super resolution imaging of brain tissue harbors difficulties due to light scattering and the density of signals. In order to reduce out of focus signal, we take advantage of the genetic tools available in the Drosophila and have fluorescently tagged synaptic proteins expressed in only a small number of neurons. These neurons form synapses within the calyx of the mushroom body, a distinct brain region involved in associative memory formation. Our results show that super resolution microscopy, in combination with genetically labeled synaptic proteins, is a powerful tool to investigate synapses in a quantitative fashion providing an entry point for studies on synaptic plasticity during learning and memory formation.
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