ArticleProceedings of the National Academy of Sciences of the United States of America2016
Longitudinal multiparameter assay of lymphocyte interactions from onset by microfluidic cell pairing and culture.
Article in Proceedings of the National Academy of Sciences of the United States of America, 2016. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 43 papers.
What it found
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
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Who cites it
43 citing papers in PubMed, 90 citations in OpenAlex.
- From single cell analysis to 3D micro physiological systems: microfluidic tools integrating cancer cell targets for delineating natural killer cell biology.Microsystems & nanoengineering · 2026Review
- A high-throughput modular microfluidic platform for versatile functional assays at single-cell level.Microsystems & nanoengineering · 2026Article
- Scalable longitudinal imaging and transcriptomics of cells in dynamic enclosures.bioRxiv : the preprint server for biology · 2026Article
- Selective access to individual cells after assembling for advanced cell-cell interaction studies.Journal of nanobiotechnology · 2026Article
- CellTrap: an instrument-free microfluidic platform for cell-cell interactions at stochastically generated effector-to-target ratios.RSC advances · 2026Article
- Gut-on-a-chip platforms: Bridging in vitro and in vivo models for advanced gastrointestinal research.Physiological reports · 2025Review
- Single Cell-Pair Proteomics for Decoding Immune-Cancer Cell Interactions.Advanced science (Weinheim, Baden-Wurttemberg, Germany) · 2025Article
- High-Throughput Single-Cell Analysis of Local Nascent Protein Deposition in 3D Microenvironments via Extracellular Protein Identification Cytometry (EPIC).Advanced materials (Deerfield Beach, Fla.) · 2025Article
- Profiling signaling mediators for cell-cell interactions and communications with microfluidics-based single-cell analysis tools.iScience · 2025Review
- Identification, sorting and profiling of functional killer cells via the capture of fluorescent target-cell lysate.Nature biomedical engineering · 2024Article
- Probing T-cell activation in nanoliter tumor co-cultures using membrane displacement trap arrays.Integrative biology : quantitative biosciences from nano to macro · 2024Article
- Cellular immunity analysis by a modular acoustofluidic platform: CIAMAP.Science advances · 2023Article
- Microfluidic device combining hydrodynamic and dielectrophoretic trapping for the controlled contact between single micro-sized objects and application to adhesion assays.Lab on a chip · 2023Article
- Microinjection inBiomicrofluidics · 2023Article
- Droplets microfluidics platform-A tool for single cell research.Frontiers in bioengineering and biotechnology · 2023Review
- Cell pairing for biological analysis in microfluidic devices.Biomicrofluidics · 2022Review
- Droplet Microfluidic Technology for the Early and Label-Free Isolation of Highly-Glycolytic, Activated T-Cells.Micromachines · 2022Article
- Assessment of chimeric antigen receptor T cytotoxicity by droplet microfluidics in vitro.Antibody therapeutics · 2022Article
- In-situ transfer vat photopolymerization for transparent microfluidic device fabrication.Nature communications · 2022Article
- Magnetophoretic Micro-Distributor for Controlled Clustering of Cells.Advanced science (Weinheim, Baden-Wurttemberg, Germany) · 2022Article
Corrections and comments
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Authors and funding
6 authors at 3 institutions in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Resolving how the early signaling events initiated by cell-cell interactions are transduced into diverse functional outcomes necessitates correlated measurements at various stages. Typical approaches that rely on bulk cocultures and population-wide correlations, however, only reveal these relationships broadly at the population level, not within each individual cell. Here, we present a microfluidics-based cell-cell interaction assay that enables longitudinal investigation of lymphocyte interactions at the single-cell level through microfluidic cell pairing, on-chip culture, and multiparameter assays, and allows recovery of desired cell pairs by micromanipulation for off-chip culture and analyses. Well-defined initiation of interactions enables probing cellular responses from the very onset, permitting single-cell correlation analyses between early signaling dynamics and later-stage functional outcomes within same cells. We demonstrate the utility of this microfluidic assay with natural killer cells interacting with tumor cells, and our findings suggest a possible role for the strength of early calcium signaling in selective coordination of subsequent cytotoxicity and IFN-gamma production. Collectively, our experiments demonstrate that this new approach is well-suited for resolving the relationships between complex immune responses within each individual cell.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.