ArticlePlant physiology2016
Molecular Evolution and Functional Characterization of a Bifunctional Decarboxylase Involved in Lycopodium Alkaloid Biosynthesis.
Article in Plant physiology, 2016. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 20 papers.
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20 citing papers in PubMed, 56 citations in OpenAlex.
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- Development of phytochemical genomics: From decoding metabolome to functional genomics and biotechnology of plant metabolism.Proceedings of the Japan Academy. Series B, Physical and biological sciences · 2025Review
- Emerging functions within the enzyme families of plant alkaloid biosynthesis.Phytochemistry reviews : proceedings of the Phytochemical Society of Europe · 2023Article
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- Assembling neuroactive alkaloids.Nature plants · 2023Article
- The first homosporous lycophyte genome revealed the association between the recent dynamic accumulation of LTR-RTs and genome size variation.Plant molecular biology · 2023Article
- Using interdisciplinary, phylogeny-guided approaches to understand the evolution of plant metabolism.Plant molecular biology · 2022Review
- Application of metabolic engineering to enhance the content of alkaloids in medicinal plants.Metabolic engineering communications · 2022Review
- A metabolic regulon reveals early and late acting enzymes in neuroactive Lycopodium alkaloid biosynthesis.Proceedings of the National Academy of Sciences of the United States of America · 2021Article
- Analysis of Genetic Diversity in the Traditional Chinese Medicine Plant 'Kushen' (Frontiers in plant science · 2021Article
- Metabolic diversification of nitrogen-containing metabolites by the expression of a heterologous lysine decarboxylase gene in Arabidopsis.The Plant journal : for cell and molecular biology · 2019Article
- Identification of a Novel Gene Encoding the Specialized Alanine Decarboxylase in Tea (Molecules (Basel, Switzerland) · 2019Article
- Contribution of Functional Divergence Through Copy Number Variations to the Inter-Species and Intra-Species Diversity in Specialized Metabolites.Frontiers in plant science · 2019Article
- Metabolic Characterization ofFrontiers in plant science · 2019Article
- Selection of Reference Genes for Expression Analysis in Chinese Medicinal HerbFrontiers in pharmacology · 2019Article
- Comprehensive relative quantitative metabolomics analysis of lycopodium alkaloids in different tissues ofSynthetic and systems biotechnology · 2018Article
- Global transcriptome analysis of Huperzia serrata and identification of critical genes involved in the biosynthesis of huperzine A.BMC genomics · 2017Article
- Quinolizidine Alkaloid Biosynthesis in Lupins and Prospects for Grain Quality Improvement.Frontiers in plant science · 2017Review
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11 authors at 3 institutions in 2 countries.
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No grant is acknowledged in the PubMed record.
Abstract
Lycopodium alkaloids (LAs) are derived from lysine (Lys) and are found mainly in Huperziaceae and Lycopodiaceae. LAs are potentially useful against Alzheimer's disease, schizophrenia, and myasthenia gravis. Here, we cloned the bifunctional lysine/ornithine decarboxylase (L/ODC), the first gene involved in LA biosynthesis, from the LA-producing plants Lycopodium clavatum and Huperzia serrata We describe the in vitro and in vivo functional characterization of the L. clavatum L/ODC (LcL/ODC). The recombinant LcL/ODC preferentially catalyzed the decarboxylation of l-Lys over l-ornithine (l-Orn) by about 5 times. Transient expression of LcL/ODC fused with the amino or carboxyl terminus of green fluorescent protein, in onion (Allium cepa) epidermal cells and Nicotiana benthamiana leaves, showed LcL/ODC localization in the cytosol. Transgenic tobacco (Nicotiana tabacum) hairy roots and Arabidopsis (Arabidopsis thaliana) plants expressing LcL/ODC enhanced the production of a Lys-derived alkaloid, anabasine, and cadaverine, respectively, thus, confirming the function of LcL/ODC in plants. In addition, we present an example of the convergent evolution of plant Lys decarboxylase that resulted in the production of Lys-derived alkaloids in Leguminosae (legumes) and Lycopodiaceae (clubmosses). This convergent evolution event probably occurred via the promiscuous functions of the ancestral Orn decarboxylase, which is an enzyme involved in the primary metabolism of polyamine. The positive selection sites were detected by statistical analyses using phylogenetic trees and were confirmed by site-directed mutagenesis, suggesting the importance of those sites in granting the promiscuous function to Lys decarboxylase while retaining the ancestral Orn decarboxylase function. This study contributes to a better understanding of LA biosynthesis and the molecular evolution of plant Lys decarboxylase.
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