ArticleAmerican journal of physiology. Endocrinology and metabolism2016
Accuracy and precision of quantitative 31P-MRS measurements of human skeletal muscle mitochondrial function.
Article in American journal of physiology. Endocrinology and metabolism, 2016. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 26 papers, 1 of them a synthesis that pooled it.
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Who cites it
26 citing papers in PubMed, 1 synthesis or guideline pooled it, 32 citations in OpenAlex.
- Pooled it
- Compromised muscle energetics and intracellular O2 availability during dynamic contraction in the plantar flexor muscles of patients with systemic sclerosis.Clinical science (London, England : 1979) · 2026Article
- Bile Acids, Mitochondria, and Impaired Oxygen Extraction: A Shared Mechanistic Pathway in Fontan Circulation and MASLD-HFpEF.Pediatric cardiology · 2026Review
- Striatal dopamine and skeletal muscle energy metabolism in older adults.The journals of gerontology. Series A, Biological sciences and medical sciences · 2026Article
- pH-Resolved ATP Synthesis in Skeletal Muscle: Concept, Implementation, and Assessment Using DynamicDiagnostics (Basel, Switzerland) · 2026Article
- Bioenergetic profiles and respiratory control in mitochondrial physiology: Precision analysis of oxidative phosphorylation.Experimental physiology · 2026Article
- Altered acute metabolic response to muscle stimulation in muscular dystrophic mice observed by phosphorus-31 magnetic resonance spectroscopy and fingerprinting.Journal of applied physiology (Bethesda, Md. : 1985) · 2025Article
- Measurement of Twitch Dynamics in Response to Exercise Induced Changes in Mitochondrial Disease Using Motor Unit Magnetic Resonance Imaging (MUMRI): A Proof-of-Concept Study.NMR in biomedicine · 2025Article
- HyperpolarizedNMR in biomedicine · 2025Article
- Reduced oxidative capacity of skeletal muscle mitochondria IS a fundamental consequence of adult ageing.The Journal of physiology · 2025Article
- Analyses of mitochondrial metabolism in diseases: a review onRSC advances · 2024Review
- Mitochondrial free radicals contribute to cigarette smoke condensate-induced impairment of oxidative phosphorylation in the skeletal muscle in situ.Free radical biology & medicine · 2024Article
- Childhood adverse life events and skeletal muscle mitochondrial function.Science advances · 2024Article
- Postcontraction [acetylcarnitine] reflects interindividual variation in skeletal muscle ATP production patterns in vivo.American journal of physiology. Regulatory, integrative and comparative physiology · 2024Article
- Cardiac and intramuscular adaptations following short-term exercise prehabilitation in unfit patients scheduled to undergo hepatic or pancreatic surgery: study protocol of a multinuclear MRI study.BMJ open gastroenterology · 2023Article
- Tissue-specific mitochondrial toxicity of cigarette smoke concentrate: consequence to oxidative phosphorylation.American journal of physiology. Heart and circulatory physiology · 2023Article
- Invasive and noninvasive markers of human skeletal muscle mitochondrial function.Physiological reports · 2023Article
- ATP and NADNutrients · 2023Article
- Multinuclear MRI in Drug Discovery.Molecules (Basel, Switzerland) · 2022Review
- Influence of NAFLD and bariatric surgery on hepatic and adipose tissue mitochondrial biogenesis and respiration.Nature communications · 2022Article
Corrections and comments
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Authors and funding
9 authors at 4 institutions in 2 countries.
Funding
Abstract
Although theoretically sound, the accuracy and precision of (31)P-magnetic resonance spectroscopy ((31)P-MRS) approaches to quantitatively estimate mitochondrial capacity are not well documented. Therefore, employing four differing models of respiratory control [linear, kinetic, and multipoint adenosine diphosphate (ADP) and phosphorylation potential], this study sought to determine the accuracy and precision of (31)P-MRS assessments of peak mitochondrial adenosine-triphosphate (ATP) synthesis rate utilizing directly measured peak respiration (State 3) in permeabilized skeletal muscle fibers. In 23 subjects of different fitness levels, (31)P-MRS during a 24-s maximal isometric knee extension and high-resolution respirometry in muscle fibers from the vastus lateralis was performed. Although significantly correlated with State 3 respiration (r = 0.72), both the linear (45 ± 13 mM/min) and phosphorylation potential (47 ± 16 mM/min) models grossly overestimated the calculated in vitro peak ATP synthesis rate (P < 0.05). Of the ADP models, the kinetic model was well correlated with State 3 respiration (r = 0.72, P < 0.05), but moderately overestimated ATP synthesis rate (P < 0.05), while the multipoint model, although being somewhat less well correlated with State 3 respiration (r = 0.55, P < 0.05), most accurately reflected peak ATP synthesis rate. Of note, the PCr recovery time constant (τ), a qualitative index of mitochondrial capacity, exhibited the strongest correlation with State 3 respiration (r = 0.80, P < 0.05). Therefore, this study reveals that each of the (31)P-MRS data analyses, including PCr τ, exhibit precision in terms of mitochondrial capacity. As only the multipoint ADP model did not overstimate the peak skeletal muscle mitochondrial ATP synthesis, the multipoint ADP model is the only quantitative approach to exhibit both accuracy and precision.
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