ArticleScientific reports2016
Fast and high resolution single-cell BRET imaging.
Article in Scientific reports, 2016. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 22 papers.
What it found
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
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Who cites it
22 citing papers in PubMed, 48 citations in OpenAlex.
- Real-Time Visualization of Isoform-Specific RAF-KRAS Interactions in Living Cells Using FRET-BRET Hybrid Biosensors.Advanced science (Weinheim, Baden-Wurttemberg, Germany) · 2026Article
- Signal Transduction Strategies for DNAzyme-Based Sensing and Imaging of Metal Ions in Cells andChemical & biomedical imaging · 2025Review
- Creating coveted bioluminescence colors for simultaneous multi-color bioimaging.Science advances · 2025Article
- Specific pharmacological and GNature communications · 2024Article
- Restoring glutamate receptosome dynamics at synapses rescues autism-like deficits in Shank3-deficient mice.Molecular psychiatry · 2021Article
- Metabolons, enzyme-enzyme assemblies that mediate substrate channeling, and their roles in plant metabolism.Plant communications · 2021Review
- Method for Detecting Emission Spectral Change of Bioluminescent Ratiometric Indicators by a Smartphone.Methods in molecular biology (Clifton, N.J.) · 2021Article
- Advanced Bioluminescence System for In Vivo Imaging with Brighter and Red-Shifted Light Emission.International journal of molecular sciences · 2020Review
- AIMTOR, a BRET biosensor for live imaging, reveals subcellular mTOR signaling and dysfunctions.BMC biology · 2020Article
- D1-mGlu5 heteromers mediate noncanonical dopamine signaling in Parkinson's disease.The Journal of clinical investigation · 2020Article
- Illuminating the kinome: Visualizing real-time kinase activity in biological systems using genetically encoded fluorescent protein-based biosensors.Current opinion in chemical biology · 2020Review
- Development and Applications of Bioluminescent and Chemiluminescent Reporters and Biosensors.Annual review of analytical chemistry (Palo Alto, Calif.) · 2019Review
- A Bioluminescence Resonance Energy Transfer-Based Approach for Determining Antibody-Receptor Occupancy In Vivo.iScience · 2019Article
- Bioluminescence resonance energy transfer-based imaging of protein-protein interactions in living cells.Nature protocols · 2019Article
- NanoBRET: The Bright Future of Proximity-Based Assays.Frontiers in bioengineering and biotechnology · 2019Review
- Combining Optical Approaches with Human Inducible Pluripotent Stem Cells in G Protein-Coupled Receptor Drug Screening and Development.Biomolecules · 2018Review
- Luciferase-induced photoreductive uncaging of small-molecule effectors.Nature communications · 2018Article
- A platform of BRET-FRET hybrid biosensors for optogenetics, chemical screening, and in vivo imaging.Scientific reports · 2018Article
- Article
- Using nanoBRET and CRISPR/Cas9 to monitor proximity to a genome-edited protein in real-time.Scientific reports · 2017Article
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
4 authors at 4 institutions in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Resonance Energy Transfer (RET)-based technologies are used to report protein-protein interactions in living cells. Among them, Bioluminescence-initiated RET (BRET) provides excellent sensitivity but the low light intensity intrinsic to the bioluminescent process hampers its use for the localization of protein complexes at the sub-cellular level. Herein we have characterized the methodological conditions required to reliably perform single-cell BRET imaging using an extremely bright luciferase, Nanoluciferase (Nluc). With this, we achieved an unprecedented performance in the field of protein-protein interaction imaging in terms of temporal and spatial resolution, duration of signal stability, signal sensitivity and dynamic range. As proof-of-principle, an Nluc-containing BRET-based sensor of ERK activity enabled the detection of subtle, transient and localized variations in ERK activity in neuronal dendritic spines, induced by the activation of endogenous synaptic NMDA receptors. This development will improve our comprehension of both the spatio-temporal dynamics of protein-protein interactions and the activation patterns of specific signaling pathways.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.