Evidence map›Paper›PMID 27302678›Full record

ArticleExperimental neurology2016

Role of PDGF-D and PDGFR-β in neuroinflammation in experimental ICH mice model.

Peng Yang, Anatol Manaenko, Feng Xu, Liyan Miao, Gaiqing Wang, Xuezhen Hu, Zhen-Ni Guo, Qin Hu, Richard E Hartman, William J Pearce and 4 more

Open access · greenAbstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in Experimental neurology, 2016. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 38 papers.

0numbers the graph read from it
0cells of the map it votes in
38citing papers in PubMed
4.0field-weighted citation impact, top 6% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

38 citing papers in PubMed, 66 citations in OpenAlex.

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  14. Platelet derived growth factor and its receptor in intracerebral hemorrhage.Zhejiang da xue xue bao. Yi xue ban = Journal of Zhejiang University. Medical sciences · 2022
    Review
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4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

14 authors at 7 institutions in 3 countries.

Peng YangDepartment of Emergency Surgery, The First Affiliated Hospital of Soochow University, Suzhou, Jiangsu, China; Departments of Physiology, Loma Linda University, Loma Linda, CA, USA. Electronic address: yangpeng@suda.edu.cn.
Anatol ManaenkoDepartments of Physiology, Loma Linda University, Loma Linda, CA, USA; Departments of Neurology, University of Erlangen-Nuremberg, Erlangen, Germany. Electronic address: anatolmanaenko@gmail.com.
Feng XuDepartment of Emergency Surgery, The First Affiliated Hospital of Soochow University, Suzhou, Jiangsu, China. Electronic address: sz_xf@suda.edu.cn.
Liyan MiaoDepartments of Pharmacy, The First Affiliated Hospital of Soochow University, Suzhou, Jiangsu, China. Electronic address: miaolysuzhou@163.com.
Gaiqing WangDepartments of Physiology, Loma Linda University, Loma Linda, CA, USA; Department of Neurology, The Second Hospital of Shanxi Medical University, Taiyuan, Shanxi, China. Electronic address: wanggq08@163.com.
Xuezhen HuDepartment of Emergency Medicine, The Second Affiliated Hospital & Yuying Children's Hospital of Wenzhou Medical University, Wenzhou, Zhejiang, China. Electronic address: 649321276@qq.com.
Zhen-Ni GuoDepartments of Physiology, Loma Linda University, Loma Linda, CA, USA; Neuroscience Center, Department of Neurology, The First Norman Bethune Hospital of Jilin University, Chang Chun, Jilin, China. Electronic address: zhen1ni2@163.com.
Qin HuDepartments of Physiology, Loma Linda University, Loma Linda, CA, USA. Electronic address: huqinle@gmail.com.
Richard E HartmanDepartments of Psychology, Loma Linda University, Loma Linda, CA, USA. Electronic address: behavioralneuroscience@gmail.com.
William J PearceDepartments of Physiology, Loma Linda University, Loma Linda, CA, USA. Electronic address: wpearce@llu.edu.
Andre ObenausDepartments of Pediatrics, Loma Linda University, Loma Linda, CA, USA. Electronic address: aobenaus@llu.edu.
John H ZhangDepartments of Physiology, Loma Linda University, Loma Linda, CA, USA; Departments of Anesthesiology, Loma Linda University, Loma Linda, CA, USA; Departments of Neurosurgery, Loma Linda University, Loma Linda, CA, USA. Electronic address: johnzhang3910@yahoo.com.
Gang ChenDepartments of Neurosurgery, The First Affiliated Hospital of Soochow University, Suzhou, Jiangsu, China. Electronic address: nju_neurosurgery@163.com.
Jiping TangDepartments of Physiology, Loma Linda University, Loma Linda, CA, USA. Electronic address: jtang@llu.edu.
Loma Linda University · USFirst Affiliated Hospital of Soochow University · CNSoochow University · CNFirst Bethune Hospital of Jilin University · CNFriedrich-Alexander-Universität Erlangen-Nürnberg · DESecond Affiliated Hospital & Yuying Children's Hospital of Wenzhou Medical University · CNShanxi Medical University · CN

Funding

Vascular Biology Core FacilityP01NS082184 · NINDS · LOMA LINDA UNIVERSITY · PI ZHANG, JOHN H · 2014 to 2018
$6.1M
Harnessing Endogenous Neuroprotection Following ICHR01NS091042 · NINDS · LOMA LINDA UNIVERSITY · PI ZHANG, JOHN H · 2016 to 2020
$1.7M
Cerebrovascular Myosin Light Chain Phosphorylation in Fetus, Newborn, and AdultR01NS076945 · NINDS · LOMA LINDA UNIVERSITY · PI PEARCE, WILLIAM J. · 2011 to 2015
$1.5M
NINDS NIH HHS P01 NS082184NINDS NIH HHS R01 NS076945NINDS NIH HHS R01 NS091042
6 · The paper itself

Abstract

objectiveInflammation plays a key role in the pathophysiological processes after intracerebral hemorrhage (ICH). Post-ICH macrophages infiltrate the brain and release pro-inflammatory factors (tumor necrosis factor-α), amplifying microglial activation and neutrophil infiltration. Platelet-derived growth factor receptor-β (PDGFR-β) is expressed on macrophages and it's activation induces the recruitment of macrophages. Platelet-derived growth factor-D (PDGF-D) is an agonist with a significantly higher affinity to the PDGFR-β compared to another isoform of the receptor. In this study, we investigated the role of PDGF-D in the pro-inflammatory response after ICH in mice.

methodsA blood injection model of ICH was used in eight-week old male CD1 mice (weight 30g). Some mice received an injection of plasmin or PDGF-D. Gleevec, a PDGFR inhibitor, was administered at 1, 3 or 6h post-ICH. Plasmin was administered with or without PDGF-D siRNAs mixture or scramble siRNA. A plasmin-antagonist, ε-Aminocaproic acid (EACA), was co-administrated with the blood. The effects of ICH and treatment on the brain injury and post-ICH inflammation were investigated.

resultsICH resulted in the overexpression of PDGF-D, associated with the infiltration of macrophages. PDGFR-inhibition decreased ICH-induced brain injury, attenuating macrophage and neutrophil infiltration, reducing microglial activation and TNF-α production. Administration of recombinant PDGF-D induced TNF-α production, and PDGFR-inhibition attenuated it. A plasmin-antagonist suppressed PDGFR-β activation and microglial activation. Plasmin increased PDGF-D expression, and PDGF-D inhibition reduced neutrophil infiltration.

conclusionICH-induced PDGF-D accumulation contributed to post-ICH inflammation via PDGFR activation and enhanced macrophage infiltration. The inhibition of PDGFR had an anti-inflammatory effect. Plasmin is a possible upstream effector of PDGF-D. The targeting of PDGF-D may provide a novel way to decrease brain injury after ICH.

Indexed as

Aminocaproic AcidAnimalsBasal GangliaBrain EdemaCerebral HemorrhageDisease Models, AnimalEncephalitisExploratory BehaviorFibrinolysinFibrinolytic AgentsGene Expression RegulationImatinib MesylateMacrophagesMaleMiceMicrogliaAminocaproic AcidFibrinolysinFibrinolytic AgentsImatinib MesylateNerve Tissue ProteinsPlatelet-Derived Growth FactorProtein Kinase InhibitorsReceptor, Platelet-Derived Growth Factor betaRNA, Small InterferingICHNeuroinflammationPDGF-DPDGFR-β

Identifiers

PMID27302678
PMCPMC5175487
OpenAlexW2419425947

What OpenQuestion holds

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Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.