ArticleNature communications2016
Genome editing in butterflies reveals that spalt promotes and Distal-less represses eyespot colour patterns.
Article in Nature communications, 2016. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 56 papers.
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Who cites it
56 citing papers in PubMed.
- Genetic parallelism underpins convergent mimicry coloration in Lepidoptera across 120 million years of evolution.PLoS biology · 2026Article
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- Whole genomes from the extinct Xerces Blue butterfly can help identify declining insect species.eLife · 2024Article
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- Comprehensive Identification and Characterization of HML-9 Group in Chimpanzee Genome.Viruses · 2024Article
- Parthenogenetic Stick Insects Exhibit Signatures of Preservation in the Molecular Architecture of Male Reproduction.Genome biology and evolution · 2024Article
- A new dawn for the study of cell type evolution.Development (Cambridge, England) · 2024Review
- Eyespot variation and field temperature in the Meadow Brown butterfly.Ecology and evolution · 2024Article
- Protein Delivery to Insect Epithelial Cells In Vivo: Potential Application to Functional Molecular Analysis of Proteins in Butterfly Wing Development.Biotech (Basel (Switzerland)) · 2023Article
- Chromosome-Level Genome Assembly ofInsects · 2023Article
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- Melanin Synthesis Pathway Interruption: CRISPR/Cas9-mediated Knockout of dopa decarboxylase (DDC) in Harmonia axyridis (Coleoptera: Coccinellidae).Journal of insect science (Online) · 2022Article
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- Cas9-mediated gene editing in the black-legged tick,iScience · 2022Article
- Butterfly eyespots evolved via cooption of an ancestral gene-regulatory network that also patterns antennae, legs, and wings.Proceedings of the National Academy of Sciences of the United States of America · 2022Article
- Transgenesis in the acoel worm Hofstenia miamia.Developmental cell · 2021Article
- High-Quality Genome Assembly and Comprehensive Transcriptome of the Painted Lady Butterfly Vanessa cardui.Genome biology and evolution · 2021Article
- Developmental Transcriptomics Reveals a Gene Network Driving Mimetic Color Variation in a Bumble Bee.Genome biology and evolution · 2021Article
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2 authors.
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Abstract
Butterfly eyespot colour patterns are a key example of how a novel trait can appear in association with the co-option of developmental patterning genes. Little is known, however, about how, or even whether, co-opted genes function in eyespot development. Here we use CRISPR/Cas9 genome editing to determine the roles of two co-opted transcription factors that are expressed during early eyespot determination. We found that deletions in a single gene, spalt, are sufficient to reduce or completely delete eyespot colour patterns, thus demonstrating a positive regulatory role for this gene in eyespot determination. Conversely, and contrary to previous predictions, deletions in Distal-less (Dll) result in an increase in the size and number of eyespots, illustrating a repressive role for this gene in eyespot development. Altogether our results show that the presence, absence and shape of butterfly eyespots can be controlled by the activity of two co-opted transcription factors.
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