ArticleNucleic acids research2016
MyoD reprogramming requires Six1 and Six4 homeoproteins: genome-wide cis-regulatory module analysis.
Article in Nucleic acids research, 2016. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 20 papers.
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Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
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Who cites it
20 citing papers in PubMed, 33 citations in OpenAlex.
- When everything is a master regulator, nothing is.Molecular biology of the cell · 2025Review
- DiPRO1 distinctly reprograms muscle and mesenchymal cancer cells.EMBO molecular medicine · 2024Article
- Peak Scores Significantly Depend on the Relationships between Contextual Signals in ChIP-Seq Peaks.International journal of molecular sciences · 2024Article
- Combination of SIX4-siRNA and temozolomide inhibits the growth and migration of A-172 glioblastoma cancer cells.Naunyn-Schmiedeberg's archives of pharmacology · 2023Article
- Overlapping functions of SIX homeoproteins during embryonic myogenesis.PLoS genetics · 2023Article
- All-trans retinoic acid changes muscle fiber type via increasing GADD34 dependent on MAPK signal.Life science alliance · 2022Article
- SIX1 reprograms myogenic transcription factors to maintain the rhabdomyosarcoma undifferentiated state.Cell reports · 2022Article
- Feedback regulation of Notch signaling and myogenesis connected by MyoD-Dll1 axis.PLoS genetics · 2021Article
- A human Myogenin promoter modified to be highly active in alveolar rhabdomyosarcoma drives an effective suicide gene therapy.Cancer gene therapy · 2021Article
- Human myotube formation is determined by MyoD-Myomixer/Myomaker axis.Science advances · 2020Article
- Single-nucleus RNA-seq and FISH identify coordinated transcriptional activity in mammalian myofibers.Nature communications · 2020Article
- Dynamic changes in cis-regulatory occupancy by Six1 and its cooperative interactions with distinct cofactors drive lineage-specific gene expression programs during progressive differentiation of the auditory sensory epithelium.Nucleic acids research · 2020Article
- Article
- SIX4 promotes metastasis through STAT3 activation in breast cancer.American journal of cancer research · 2020Article
- Time-dependent Pax3-mediated chromatin remodeling and cooperation with Six4 and Tead2 specify the skeletal myogenic lineage in developing mesoderm.PLoS biology · 2019Article
- Review
- Shaping Gene Expression by Landscaping Chromatin Architecture: Lessons from a Master.Molecular cell · 2018Review
- Arginine Methylation by PRMT1 Regulates Muscle Stem Cell Fate.Molecular and cellular biology · 2017Article
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Corrections and comments
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Authors and funding
11 authors at 5 institutions in 2 countries.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Myogenic regulatory factors of the MyoD family have the ability to reprogram differentiated cells toward a myogenic fate. In this study, we demonstrate that Six1 or Six4 are required for the reprogramming by MyoD of mouse embryonic fibroblasts (MEFs). Using microarray experiments, we found 761 genes under the control of both Six and MyoD. Using MyoD ChIPseq data and a genome-wide search for Six1/4 MEF3 binding sites, we found significant co-localization of binding sites for MyoD and Six proteins on over a thousand mouse genomic DNA regions. The combination of both datasets yielded 82 genes which are synergistically activated by Six and MyoD, with 96 associated MyoD+MEF3 putative cis-regulatory modules (CRMs). Fourteen out of 19 of the CRMs that we tested demonstrated in Luciferase assays a synergistic action also observed for their cognate gene. We searched putative binding sites on these CRMs using available databases and de novo search of conserved motifs and demonstrated that the Six/MyoD synergistic activation takes place in a feedforward way. It involves the recruitment of these two families of transcription factors to their targets, together with partner transcription factors, encoded by genes that are themselves activated by Six and MyoD, including Mef2, Pbx-Meis and EBF.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.