ArticleThe Journal of biological chemistry2016
Magnetite Biomineralization in Magnetospirillum magneticum Is Regulated by a Switch-like Behavior in the HtrA Protease MamE.
Article in The Journal of biological chemistry, 2016. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 16 papers.
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Who cites it
16 citing papers in PubMed, 29 citations in OpenAlex.
- A cumate-inducible promoter system for tuned gene expression and magnetosome overproduction in magnetotactic bacteria.Applied microbiology and biotechnology · 2026Article
- Article
- Cross-linking proteomics analysis for reliable identification of MamXY-associated magnetosome formation.Brazilian journal of microbiology : [publication of the Brazilian Society for Microbiology] · 2026Article
- The Role of Confinement in Biomineralization.Chemical reviews · 2025Review
- Cellular Distribution and Motion of Essential Magnetosome Proteins Expressed in Mammalian Cells.Biosensors · 2025Article
- Magnetosome organelles are organized through interactions between McaA and McaB that alter the dynamics of the bacterial actin-like protein MamK.bioRxiv : the preprint server for biology · 2025Article
- Intrinsic and extrinsic determinants of conditional localization of Mms6 to magnetosome organelles inJournal of bacteriology · 2024Article
- Silent gene clusters encode magnetic organelle biosynthesis in a non-magnetotactic phototrophic bacterium.The ISME journal · 2023Article
- A protease-mediated switch regulates the growth of magnetosome organelles inProceedings of the National Academy of Sciences of the United States of America · 2022Article
- Large-scale survey and database of high affinity ligands for peptide recognition modules.Molecular systems biology · 2020Article
- A Compass To Boost Navigation: Cell Biology of Bacterial Magnetotaxis.Journal of bacteriology · 2020Review
- Magnetic genes: Studying the genetics of biomineralization in magnetotactic bacteria.PLoS genetics · 2020Review
- Distinct Contribution of the HtrA Protease and PDZ Domains to Its Function in Stress Resilience and Virulence ofFrontiers in microbiology · 2019Article
- Regulation of nitric oxide signaling by formation of a distal receptor-ligand complex.Nature chemical biology · 2017Article
- The Disruption of an OxyR-Like Protein Impairs Intracellular Magnetite Biomineralization inFrontiers in microbiology · 2017Article
- Magnetosome biogenesis in magnetotactic bacteria.Nature reviews. Microbiology · 2016Review
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Authors and funding
7 authors at 4 institutions in 3 countries.
Funding
Abstract
Magnetotactic bacteria are aquatic organisms that produce subcellular magnetic particles in order to orient in the earth's geomagnetic field. MamE, a predicted HtrA protease required to produce magnetite crystals in the magnetotactic bacterium Magnetospirillum magneticum AMB-1, was recently shown to promote the proteolytic processing of itself and two other biomineralization factors in vivo Here, we have analyzed the in vivo processing patterns of three proteolytic targets and used this information to reconstitute proteolysis with a purified form of MamE. MamE cleaves a custom peptide substrate with positive cooperativity, and its autoproteolysis can be stimulated with exogenous substrates or peptides that bind to either of its PDZ domains. A misregulated form of the protease that circumvents specific genetic requirements for proteolysis causes biomineralization defects, showing that proper regulation of its activity is required during magnetite biosynthesis in vivo Our results represent the first reconstitution of the proteolytic activity of MamE and show that its behavior is consistent with the previously proposed checkpoint model for biomineralization.
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