ArticleBlood2016
Full activation of mouse platelets requires ADP secretion regulated by SERCA3 ATPase-dependent calcium stores.
Article in Blood, 2016. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 22 papers.
What it found
Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.
The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.
Who cites it
22 citing papers in PubMed, 39 citations in OpenAlex.
- Multi-omics analysis reveals gut microbiota-host metabolism-shell gland axis underlying eggshell deterioration in aged laying hens.Poultry science · 2026Article
- The crucial role of intercellular calcium wave propagation triggered by influenza A virus in promoting infection.Cell communication and signaling : CCS · 2025Article
- SERCA Modulators Reveal Distinct Signaling and Functional Roles of T Lymphocyte CaInternational journal of molecular sciences · 2024Article
- The single-cell transcriptomic landscape of the topological differences in mammalian auditory receptors.Science China. Life sciences · 2024Article
- How Protein Depletion Balances Thrombosis and Bleeding Risk in the Context of Platelet's Activatory and Negative Signaling.International journal of molecular sciences · 2024Review
- ADP receptor P2Y12 is the capstone of the cross-talk between CaResearch and practice in thrombosis and haemostasis · 2023Article
- Review
- The secreted tyrosine kinase VLK is essential for normal platelet activation and thrombus formation.Blood · 2022Article
- Humic Acids Inhibit Platelet Activation to Reduce Venous Thromboembolism in Mice.Evidence-based complementary and alternative medicine : eCAM · 2022Article
- Endoglin Is an Endothelial Housekeeper against Inflammation: Insight in ECFC-Related Permeability through LIMK/Cofilin Pathway.International journal of molecular sciences · 2021Article
- Calcium-Collagen Coupling is Vital for Biomineralization Schedule.Advanced science (Weinheim, Baden-Wurttemberg, Germany) · 2021Article
- Platelets orchestrate the resolution of pulmonary inflammation in mice by T reg cell repositioning and macrophage education.The Journal of experimental medicine · 2021Article
- Elevated body mass index facilitates early postoperative complications after surgery for intracranial meningioma.Neurosurgical review · 2021Article
- Combined Metallomics/Transcriptomics Profiling Reveals a Major Role for Metals in Wound Repair.Frontiers in cell and developmental biology · 2021Article
- Endoplasmic Reticulum Calcium Pumps and Tumor Cell Differentiation.International journal of molecular sciences · 2020Review
- Article
- Primary Active CaCold Spring Harbor perspectives in biology · 2020Review
- Structure-guided design of pure orthosteric inhibitors of αIIbβ3 that prevent thrombosis but preserve hemostasis.Nature communications · 2020Article
- Article
- Glycoprotein VI in securing vascular integrity in inflamed vessels.Research and practice in thrombosis and haemostasis · 2018Article
Corrections and comments
- Commented on by
Authors and funding
8 authors at 5 institutions in 2 countries.
Funding
No grant is acknowledged in the PubMed record.
Abstract
The role of the sarco-endoplasmic reticulum calcium (Ca(2+)) adenosine triphosphatase (ATPase) 3 (SERCA3) in platelet physiology remains poorly understood. Here, we show that SERCA3 knockout (SERCA3(-/-)) mice exhibit prolonged tail bleeding time and rebleeding. Thrombus formation was delayed both in arteries and venules in an in vivo ferric chloride-induced thrombosis model. Defective platelet adhesion and thrombus growth over collagen was confirmed in vitro. Adenosine 5'-diphosphate (ADP) removal by apyrase diminished adhesion and thrombus growth of control platelets to the level of SERCA3(-/-) platelets. Aggregation, dense granule secretion, and Ca(2+) mobilization of SERCA3(-/-) platelets induced by low collagen or low thrombin concentration were weaker than controls. Accordingly, SERCA3(-/-) platelets exhibited a partial defect in total stored Ca(2+) and in Ca(2+) store reuptake following thrombin stimulation. Importantly ADP, but not serotonin, rescued aggregation, secretion, and Ca(2+) mobilization in SERCA3(-/-) platelets, suggesting specificity. Dense granules appeared normal upon electron microscopy, mepacrine staining, and total serotonin content, ruling out a dense granule defect. ADP induced normal platelet aggregation, excluding a defect in ADP activation pathways. The SERCA3-specific inhibitor 2,5-di-(tert-butyl)-1,4-benzohydroquinone diminished both Ca(2+) mobilization and secretion of control platelets, as opposed to the SERCA2b inhibitor thapsigargin. This confirmed the specific role of catalytically active SERCA3 in ADP secretion. Accordingly, SERCA3-dependent Ca(2+) stores appeared depleted in SERCA3(-/-) platelets. Finally, αIIbβ3 integrin blockade did not affect SERCA3-dependent secretion, therefore proving independent of αIIbβ3 engagement. Altogether, these results show that SERCA3-dependent Ca(2+) stores control a specific ADP secretion pathway required for full platelet secretion induced by agonists at low concentration and independent of αIIbβ3.
Indexed as
Identifiers
What OpenQuestion holds
Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.