Evidence map›Paper›PMID 27301270›Full record

ArticleNature communications2016

Printable enzyme-embedded materials for methane to methanol conversion.

Craig D Blanchette, Jennifer M Knipe, Joshuah K Stolaroff, Joshua R DeOtte, James S Oakdale, Amitesh Maiti, Jeremy M Lenhardt, Sarah Sirajuddin, Amy C Rosenzweig, Sarah E Baker

Open access · goldAbstract read
In one paragraph

Article in Nature communications, 2016. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 14 papers.

0numbers the graph read from it
0cells of the map it votes in
14citing papers in PubMed
6.1field-weighted citation impact, top 3% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

14 citing papers in PubMed, 105 citations in OpenAlex.

  1. Review
  2. Review
  3. Article
  4. Article
  5. Article
  6. Atmospheric methane removal: a research agenda.Philosophical transactions. Series A, Mathematical, physical, and engineering sciences · 2021
    Article
  7. Article
  8. Review
  9. Article
  10. Article
  11. Additive Manufacturing: Unlocking the Evolution of Energy Materials.Advanced science (Weinheim, Baden-Wurttemberg, Germany) · 2017
    Review
  12. Review
  13. Review
  14. Article
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

10 authors at 2 institutions in 1 country.

Craig D BlanchetteLawrence Livermore National Laboratory, 7000 East Avenue, Livermore, California 94551, USA.
Jennifer M KnipeLawrence Livermore National Laboratory, 7000 East Avenue, Livermore, California 94551, USA.
Joshuah K StolaroffLawrence Livermore National Laboratory, 7000 East Avenue, Livermore, California 94551, USA.
Joshua R DeOtteLawrence Livermore National Laboratory, 7000 East Avenue, Livermore, California 94551, USA.
James S OakdaleLawrence Livermore National Laboratory, 7000 East Avenue, Livermore, California 94551, USA.
Amitesh MaitiLawrence Livermore National Laboratory, 7000 East Avenue, Livermore, California 94551, USA.
Jeremy M LenhardtLawrence Livermore National Laboratory, 7000 East Avenue, Livermore, California 94551, USA.
Sarah SirajuddinDepartment of Molecular Biosciences and of Chemistry, Northwestern University, Evanston, Illinois 60208, USA.
Amy C RosenzweigDepartment of Molecular Biosciences and of Chemistry, Northwestern University, Evanston, Illinois 60208, USA.
Sarah E BakerLawrence Livermore National Laboratory, 7000 East Avenue, Livermore, California 94551, USA.
Lawrence Livermore National Laboratory · USNorthwestern University · US

Funding

Metalloenzymes and metal homeostasisR35GM118035 · NIGMS · NORTHWESTERN UNIVERSITY · PI AMY C. ROSENZWEIG · 2016 to 2026
$7.1M
Particulate Methane MonooxygenaseR01GM070473 · NIGMS · NORTHWESTERN UNIVERSITY · PI ROSENZWEIG, AMY C. · 2004 to 2015
$3.7M
NIGMS NIH HHS R01 GM070473NIGMS NIH HHS R35 GM118035
6 · The paper itself

Abstract

An industrial process for the selective activation of methane under mild conditions would be highly valuable for controlling emissions to the environment and for utilizing vast new sources of natural gas. The only selective catalysts for methane activation and conversion to methanol under mild conditions are methane monooxygenases (MMOs) found in methanotrophic bacteria; however, these enzymes are not amenable to standard enzyme immobilization approaches. Using particulate methane monooxygenase (pMMO), we create a biocatalytic polymer material that converts methane to methanol. We demonstrate embedding the material within a silicone lattice to create mechanically robust, gas-permeable membranes, and direct printing of micron-scale structures with controlled geometry. Remarkably, the enzymes retain up to 100% activity in the polymer construct. The printed enzyme-embedded polymer motif is highly flexible for future development and should be useful in a wide range of applications, especially those involving gas-liquid reactions.

Indexed as

BioprintingBioreactorsEnzyme StabilityHydrogel, Polyethylene Glycol DimethacrylateMethaneMethanolMethylococcusOxygenasesParticulate MatterPolyethylene GlycolsHydrogel, Polyethylene Glycol DimethacrylateMethanemethane monooxygenaseMethanolOxygenasesParticulate Matterpoly(ethylene glycol)diacrylatePolyethylene Glycols

Identifiers

PMID27301270
PMCPMC4912616
OpenAlexW2431792572

What OpenQuestion holds

Textmetadata
LicenceCC BY
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.