ArticleEndocrinology2016
T3 Regulates a Human Macrophage-Derived TSH-β Splice Variant: Implications for Human Bone Biology.
Article in Endocrinology, 2016. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 20 papers.
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
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Who cites it
20 citing papers in PubMed, 58 citations in OpenAlex.
- Identification of TSHB gene expression profile in Duolang sheep and its functional role in granulosa cells.BMC genomics · 2026Article
- Molecular and Functional Insights into Thyrostimulin and Its Subunits-GPA2/GPB5.International journal of molecular sciences · 2025Review
- Neuroendocrinology of bone.Pituitary · 2024Review
- Pituitary crosstalk with bone, adipose tissue and brain.Nature reviews. Endocrinology · 2023Review
- Article
- Independent Skeletal Actions of Pituitary Hormones.Endocrinology and metabolism (Seoul, Korea) · 2022Article
- Article
- Triiodothyronine (T3) Induces Limited Transcriptional and DNA Methylation Reprogramming in Human Monocytes.Biomedicines · 2022Article
- Thyrotropin, Hyperthyroidism, and Bone Mass.The Journal of clinical endocrinology and metabolism · 2021Review
- Thyroid-stimulating hormone decreases the risk of osteoporosis by regulating osteoblast proliferation and differentiation.BMC endocrine disorders · 2021Article
- Pathways involved in pony body size development.BMC genomics · 2021Article
- Dynamic Interactions Between the Immune System and the Neuroendocrine System in Health and Disease.Frontiers in endocrinology · 2021Review
- Unconventional Actions of Glycoprotein Hormone Subunits: A Comprehensive Review.Frontiers in endocrinology · 2021Review
- Beyond bone biology: Lessons from team science.Journal of orthopaedic research : official publication of the Orthopaedic Research Society · 2020Article
- The Human TSHβ Subunit Proteins and Their Binding Sites on the TSH Receptor Using Molecular Dynamics Simulation.Endocrinology · 2020Article
- Thyroid stimulating hormone β-subunit splice variant is expressed in all fractional subsets of bone marrow hematopoietic cells and peripheral blood leukocytes and is modulated during bacterial infection.General and comparative endocrinology · 2020Article
- Novel Splicing of Immune System Thyroid Stimulating Hormone β-Subunit-Genetic Regulation and Biological Importance.Frontiers in endocrinology · 2019Review
- Regulation of Skeletal Homeostasis.Endocrine reviews · 2018Review
- Actions of pituitary hormones beyond traditional targets.The Journal of endocrinology · 2018Review
- Expanding the Role of Thyroid-Stimulating Hormone in Skeletal Physiology.Frontiers in endocrinology · 2017Review
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Authors and funding
5 authors at 2 institutions in 1 country.
Funding
Abstract
TSH and thyroid hormones (T3 and T4) are intimately involved in bone biology. We have previously reported the presence of a murine TSH-β splice variant (TSH-βv) expressed specifically in bone marrow-derived macrophages and that exerted an osteoprotective effect by inducing osteoblastogenesis. To extend this observation and its relevance to human bone biology, we set out to identify and characterize a TSH-β variant in human macrophages. Real-time PCR analyses using human TSH-β-specific primers identified a 364-bp product in macrophages, bone marrow, and peripheral blood mononuclear cells that was sequence verified and was homologous to a human TSH-βv previously reported. We then examined TSH-βv regulation using the THP-1 human monocyte cell line matured into macrophages. After 4 days, 46.1% of the THP-1 cells expressed the macrophage markers CD-14 and macrophage colony-stimulating factor and exhibited typical morphological characteristics of macrophages. Real-time PCR analyses of these cells treated in a dose-dependent manner with T3 showed a 14-fold induction of human TSH-βv mRNA and variant protein. Furthermore, these human TSH-βv-positive cells, induced by T3 exposure, had categorized into both M1 and M2 macrophage phenotypes as evidenced by the expression of macrophage colony-stimulating factor for M1 and CCL-22 for M2. These data indicate that in hyperthyroidism, bone marrow resident macrophages have the potential to exert enhanced osteoprotective effects by oversecreting human TSH-βv, which may exert its local osteoprotective role via osteoblast and osteoclast TSH receptors.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.