ArticlePloS one2016
Validation of Reference Genes for Gene Expression by Quantitative Real-Time RT-PCR in Stem Segments Spanning Primary to Secondary Growth in Populus tomentosa.
Article in PloS one, 2016. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 18 papers.
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Who cites it
18 citing papers in PubMed.
- Selection and Validation of qRT-PCR Reference Genes inCurrent issues in molecular biology · 2026Article
- Vein-Specific Overexpression of Galactinol Synthase Gene Improves Salinity Tolerance in Poplar.Plant, cell & environment · 2026Article
- Integrated multi-omics analysis reveals the molecular mechanism underlying poplar 107 rootstock-mediated regulation ofHorticulture research · 2026Article
- Identification and Validation of Reference Genes for Reliable RT-qPCR Normalization inPlants (Basel, Switzerland) · 2026Article
- Article
- A protocol for identifying universal reference genes within a genus based on RNA-Seq data: a case study of poplar stem gene expression.Forestry research · 2024Article
- The transcription factor MYB156 controls the polar stiffening of guard cell walls in poplar.The Plant cell · 2023Article
- Expanding the application of a UV-visible reporter for transient gene expression and stable transformation in plants.Horticulture research · 2021Article
- Isotopically Dimethyl Labeling-Based Quantitative Proteomic Analysis of Phosphoproteomes of Soybean Cultivars.Biomolecules · 2021Article
- An improved and efficient method of Agrobacterium syringe infiltration for transient transformation and its application in the elucidation of gene function in poplar.BMC plant biology · 2021Article
- A Small Guanosine Triphosphate Binding ProteinFrontiers in plant science · 2021Article
- Extensive transcriptome changes during seasonal leaf senescence in field-grown black cottonwood (Populus trichocarpa Nisqually-1).Scientific reports · 2020Article
- RNA interference suppression of AGAMOUS and SEEDSTICK alters floral organ identity and impairs floral organ determinacy, ovule differentiation, and seed-hair development in Populus.The New phytologist · 2019Article
- Endoplasmic reticulum-localized UBC34 interaction with lignin repressors MYB221 and MYB156 regulates the transactivity of the transcription factors in Populus tomentosa.BMC plant biology · 2019Article
- Reference gene selection for qRT-PCR assays inPeerJ · 2018Article
- Article
- Automated identification of reference genes based on RNA-seq data.Biomedical engineering online · 2017Article
- Selection and Validation of Appropriate Reference Genes for qRT-PCR Analysis inFrontiers in plant science · 2017Article
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Authors and funding
6 authors.
Funding
No grant is acknowledged in the PubMed record.
Abstract
The vertical segments of Populus stems are an ideal experimental system for analyzing the gene expression patterns involved in primary and secondary growth during wood formation. Suitable internal control genes are indispensable to quantitative real time PCR (qRT-PCR) assays of gene expression. In this study, the expression stability of eight candidate reference genes was evaluated in a series of vertical stem segments of Populus tomentosa. Analysis through software packages geNorm, NormFinder and BestKeeper showed that genes ribosomal protein (RP) and tubulin beta (TUBB) were the most unstable across the developmental stages of P. tomentosa stems, and the combination of the three reference genes, eukaryotic translation initiation factor 5A (eIF5A), Actin (ACT6) and elongation factor 1-beta (EF1-beta) can provide accurate and reliable normalization of qRT-PCR analysis for target gene expression in stem segments undergoing primary and secondary growth in P. tomentosa. These results provide crucial information for transcriptional analysis in the P. tomentosa stem, which may help to improve the quality of gene expression data in these vertical stem segments, which constitute an excellent plant system for the study of wood formation.
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