ArticleFood and environmental virology2016
The Effect of Heat on the Physicochemical Properties of Bacteriophage MS2.
Article in Food and environmental virology, 2016. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 13 papers.
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
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Who cites it
13 citing papers in PubMed, 41 citations in OpenAlex.
- Bacteriophage Challenges in Industrial Processes: A Historical Unveiling and Future Outlook.Pathogens (Basel, Switzerland) · 2024Review
- An Overview of Diverse Strategies To InactivateEcoSal Plus · 2023Review
- Reproducibility and sensitivity of 36 methods to quantify the SARS-CoV-2 genetic signal in raw wastewater: findings from an interlaboratory methods evaluation in the U.S.Environmental science : water research & technology · 2021Article
- Free Chlorine and Peroxynitrite Alter the Capsid Structure of Human Norovirus GII.4 and Its Capacity to Bind Histo-Blood Group Antigens.Frontiers in microbiology · 2021Article
- The effect of proteolytic enzymes and pH on GII.4 norovirus, during both interactions and non-interaction with Histo-Blood Group Antigens.Scientific reports · 2020Article
- The Basis of Peracetic Acid Inactivation Mechanisms for Rotavirus and Tulane Virus under Conditions Relevant for Vegetable Sanitation.Applied and environmental microbiology · 2020Article
- Hydrophobic Organic Matter Promotes Coxsackievirus B5 Stabilization and Protection from Heat.Food and environmental virology · 2020Article
- Effect of natural ageing and heat treatments on GII.4 norovirus binding to Histo-Blood Group Antigens.Scientific reports · 2019Article
- Influence of physico-chemical characteristics of sediment on the in situ spatial distribution of F-specific RNA phages in the riverbed.FEMS microbiology ecology · 2019Article
- Temperature-Dependent Nanomechanics and Topography of Bacteriophage T7.Journal of virology · 2018Article
- Effect of the Shellfish Proteinase K Digestion Method on Norovirus Capsid Integrity.Food and environmental virology · 2018Article
- Development of an efficient viral aerosol collector for higher sampling flow rate.Environmental science and pollution research international · 2018Article
- The Effect of Heat and Free Chlorine Treatments on the Surface Properties of Murine Norovirus.Food and environmental virology · 2017Article
Corrections and comments
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Authors and funding
5 authors at 2 institutions in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
The differences in physicochemical characteristics between infectious and non-infectious viral particles are poorly known. Even for heat, which is known as one of the most efficient treatments to inactivate enteric viruses, the global inactivation mechanisms have not been described yet. Such knowledge would help distinguish between both types of particles and therefore clarify the interpretation of the presence of viral genomes in food after heat treatment. In this study, we examined in particular the differences in electrostatic charge and hydrophobicity between the two particle types. MS2 phage, a common surrogate for enteric viruses, was used as a model virus. The heat-induced inactivation process of the infectious phages caused hydrophobic domains to be transiently exposed and their charge to become less negative. The particles also became progressively permeable to small molecules such as SYPRO Orange dye. The presence of non-infectious phage particles in which the genome was not accessible to RNases has been clearly demonstrated. These observations were done for MS2 phages exposed to a temperature of 60 °C. When exposed to a temperature higher than their critical temperature (72 °C), the particles were disrupted and the genome became available for RNases. At lower temperatures, 60 °C in this study, the transient expression of hydrophobic domains of remaining infectious phages appeared as an interesting parameter for improving their specific detection.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.